Type of application | Rationale | Observations | Amphipols used | References |
MP immobilization onto solid supports | Tagged APols will simultaneously make a MP water-soluble, stabilize it biochemically, and anchor it onto a solid support. | Tags that have been validated to date include biotin, polyhistidine, an oligodeoxynucleotide, and distributed imidazole moieties. | A8-35, NAPols, PC-APols |
Basit et al. (2012), Charvolin et al. (2009), Della Pia et al. (2014a, b), Ferrandez et al. (2014), Giusti et al. (2014a), Le Bon et al. (2014a) |
Delivery of MPs to preexisting membranes | APols do no lyse target membranes (lipid vesicles or black films, cell plasma membrane) and can therefore be used to deliver to them hydrophobic cargoes. | TMPs have been delivered to lipid vesicles, black films, mesophases, and cell plasma membranes. Caveats: insertion process expected to be traumatic for fragile MPs; carrier APols will remain associated to the target membrane. | A8-35, A8-75, PMAL |
Kyrychenko et al. (2012), Nagy et al. (2001), Pocanschi et al. (2006), Polovinkin et al. (2014b), Popot et al. (2011) |
Vaccination | Stabilizing biochemically and physically MPs used as immunogens. Co-delivering them along with APol-bound or co-trapped adjuvants. | The A8-35-trapped major outer membrane protein (MOMP) from Chlamydia trachomatis offers a much better protection to vaccinated mice than its detergent-solubilized counterpart. | A8-35 | Tifrea et al. (2011, 2014) |