Tassadite DAHMANE

Tassadite DAHMANE is a postdoctoral scientist in the department of Biochemistry and Molecular Biophysics at Columbia University, New York. Under the direction of Jean-Luc Popot, she developed the method of amphipol-assisted refolding of bacteriorhodopsin, which, in collaboration with Jean-Louis Banères, she then extended to folding G protein-coupled receptors produced as inclusion bodies.  After her Ph.D., she decided to extend her expertise to the crystallization of proteins.

She is actually working on the crystallization of multiple proteins, including membrane proteins.


Department of Biochemistry and Molecular Biophysics, Columbia University
Wayne Hendrickson laboratory
650 West 168th Street, New York, NY 10032
Mail: td2262(at)columbia.edu
Lab web page: http://convex.hhmi.columbia.edu/2010/index.html

Selected Articles:

  1. Picard, M., Dahmane, T., Garrigos, M., Gauron, C., Giusti, F., le Maire, M., Popot, J.-L. & Champeil, P. (2006). Protective and inhibitory effects of various types of amphipols on the Ca2+-ATPase from sarcoplasmic reticulum: a comparative study. Biochemistry 45, 1861-1869.
  2. Pocanschi, C. L., Dahmane, T., Gohon, Y., Rappaport, F., Apell, H.-J., Kleinschmidt, J. H. & Popot, J.-L. (2006). Amphipathic polymers: tools to fold integral membrane proteins to their active form. Biochemistry 45, 13954-13961.
  3. Gohon, Y., Dahmane, T., Ruigrok, R., Schuck, P., Charvolin, D., Rappaport, F., Timmins, P., Engelman, D. M., Tribet, C., Popot, J.-L. & Ebel, C. (2008). Bacteriorhodopsin/amphipol complexes: structural and functional properties. Biophys. J. 94, 3523-3537.
  4. Sharma, K. S., Durand, G., Giusti, F., Olivier, B., Fabiano, A.-S., Bazzacco, P., Dahmane, T., Ebel, C., Popot, J.-L. & Pucci, B. (2008). Glucose-based amphiphilic telomers designed to keep membrane proteins soluble in aqueous solutions: synthesis and physicochemical characterization. Langmuir 24, 13581-13590.
  5. Dahmane, T., Damian, M., Mary, S., Popot, J.-L. & Banères, J.-L. (2009). Amphipol-assisted in vitro folding of G protein-coupled receptors. Biochemistry 48, 6516-6521.
  6. Tribet, C., Diab, C., Dahmane, T., Zoonens, M., Popot, J.-L. & Winnik, F. M. (2009). Thermodynamic characterization of the exchange of detergents and amphipols at the surfaces of integral membrane proteins. Langmuir 25, 12623-12634.
  7. Dahmane, T., Giusti, F., Catoire, L. J. & Popot, J.-L. (2011). Sulfonated amphipols: Synthesis, properties and applications. Biopolymers 95, 811-823.
  8. Park, K.-H., Billon-Denis, E., Dahmane, T., Lebaupain, F., Pucci, B., Breyton, C. & Zito, F. (2011). In the cauldron of cell-free synthesis of membrane proteins: Playing with new surfactants. New Biotech. 28, 255-261.

Selected Reviews:

  1. Popot, J.-L., Althoff, T., Bagnard, D., Banères, J.-L., Bazzacco, P., Billon-Denis, E., Catoire, L. J., Champeil, P., Charvolin, D., Cocco, M. J., Crémel, G., Dahmane, T., de la Maza, L. M., Ebel, C., Gabel, F., Giusti, F., Gohon, Y., Goormaghtigh, E., Guittet, E., Kleinschmidt, J. H., Kühlbrandt, W., Le Bon, C., Martinez, K. L., Picard, M., Pucci, B., Rappaport, F., Sachs, J. N., Tribet, C., van Heijenoort, C., Wien, F., Zito, F. & Zoonens, M. (2011). Amphipols from A to Z. Annu. Rev. Biophys. 40, 379–408.