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• Description
• Program
  • Workshop Schedule
  • Theoretical Part
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To train industrial and academic scientists in the use of amphipols for such applications as membrane protein stabilization, folding, cell-free synthesis and immobilization, drug screening, diagnostics, and structural studies.

Audience: Students and industrial or academic scientists experienced in working with membrane proteins in vitro.

Organizer: Jean-Luc Popot, CNRS/Université Paris 7 UMR 7099.

Venue: Institut de Biologie Physico-Chimique, 13 rue Pierre-et-Marie-Curie, F-75005 PARIS, France.

Date: March 8-12, 2010.

Outline: The workshop will comprise two parts: i) a series of presentations (Theoretical part), and ii) hands-on training (Practicals).The language will be English.

Theoretical part

To be spread over one and a half day. Open to up to 40 people. Will include presentations about membrane protein structure, membrane protein stability in aqueous solutions, structure and properties of amphipols, functionalized amphipols, the use of amphipols for trapping membrane proteins, structure and properties of membrane protein/amphipol complexes, amphipol-assisted membrane protein folding, the use of amphipols for membrane protein cell-free synthesis, amphipol-mediated membrane protein immobilization and its application to drug screening and diagnostics, NMR studies of amphipol-trapped membrane proteins, proteomics.

Speakers whose participation has been confirmed.

Practicals

To be spread over the rest of the week. Open to 6-12 people depending on i) space available, and ii) number of students interested in the various types of training. Experiments will be carried out on one of the membrane proteins currently in use in the laboratory (bacteriorhodopsin). Depending on the desiderata expressed by the students upon registration, practicals will include membrane protein trapping, membrane protein folding, cell-free synthesis, characterization by SEC of membrane protein/amphipol complexes, membrane protein immobilization onto solid supports and surface plasmon resonance detection of antibody binding.

Teachers.

Staff of CNRS/Paris-7 University UMR 7099.