AMPHIPOLS: Library of labeled derivatives amphipols

A brief description of several modified amphipols (Zoonens et Popot ( 2014), Le Bon et al. (2014)).

Type of modification Applications Name of labeled derivatives amphipols Refererences
Isotopic labeling
Radioactive isotopes: 14C, 3H.

Heavy isotopes.
- Following APol distribution and exchange for other surfactants.

- Eliminating protons for NMR measurements, contrast-matching in SANS, AUC.

- Perdeuterated AmphiPol (PerDAPol)

- Deuterated AmphiPol (DAPol)
Radioactive isotopes
Tribet et al. (1997),
Gohon et al. (2008),

Heavy isotopes
Catoire et al. (2010a), Giusti et al (2014b).
Fluorescent labeling
Fluorescent dyes (λexem):

(290 nm/310-370 nm)

(470 nm/530 nm)

(495 nm/540 nm)

(555 nm/575 nm)

(651 nm/668 nm)

-Studying APol distribution, binding and exchange for other surfactants and upon delivery of a membrane protein to lipid vesicles or cells in culture.

-Mapping the transmembrane domain of a protein by FRET with single tryptophane.

-Following in vivo distribution and elimination of APol.
- Fluorescent AmphiPol (FAPol)

Figure .Normalized excitation spectra of the various fluorescent dyes (Della Pia et al. (2014 b)

Vial et al. (2005),

Giusti et al. (2012),
Zoonens et al. (2007),

Opačić et al. (2014a),

Giusti et al. (2012),
Fernandez et al. (2014),
Della Pia et al. (2014a),

Alexa Fluor 647
Fernandez et al. (2014),
Della Pia et al. (2014a),
Le Bon et al. (2014a).
Immobilization labeling
Tags, adjuvants:


-Hexahistidine peptide,

-Imidazole ring,


-Immobilizing membrane proteins onto chips or beads in a more or less reversible mode for SPR or fluorescence measurements.

- Vaccination, using the oligodeoxynucleotide as an adjuvant.
-Biotinylated amphipol (BAPol)
-Hexahistidine peptide labeled amphipol (HistAPol),
-Imidazole labeled amphipol (ImidAPol),
-ODN labeled amphipol(OligAPol).

Figure .Illustration presenting the physical properties of the immobilization tags of APols
(Della Pia et al. (2014a, b)).

Charvolin et al. (2009),
Della Pia et al.
(2014 a,b)
Fernandez et al. (2014),

Giusti et al. (in preparation),

Le Bon et al. (2014a).