- The team has just secured funding for 1 ANR project (more info to come)
- C. Tisné is keynote speaker at the next BSI conference
P. Barraud , C. Tisné (2023). Cracking the case of m7G modification in human tRNAs, Nat Struct Mol Biol doi: 10.1038/s41594-023-00937-5
M.-J. Yared, Y. Yoluç, M. Catala, C. Tisné, S. Kaiser, P. Barraud*. Different modification pathways for m1A58 incorporation in yeast elongator and initiator tRNAs. Nucleic acids Res. (2023), doi: 10.1093/nar/gkad722
V. Meynier#, L. Iannazzo#, M. Catala, S. Oerum, E. Braud, C. Adjian, P. Barraud, M. Fonvielle, C. Tisné*£, M. Etheve-Quelquejeu*£. Synthesis of RNA-Cofactor conjugates and structural exploration of RNA recognition by m6A RNA methyltransferases. Nucleic acids Res. 50 (2022), p. 5793-5806. doi: 10.1093/nar/gkac354.
S. Oerum, V. Meynier, M. Catala & C. Tisné*, A comprehensive review of m6A/m6Am RNA methyltransferase structures, Nucleic Acids Res. 49 (2021), p. 7239-7255. doi:10.1093/nar/gkab378
S. Oerum, T. Dendooven, M. Catala, L. Gilet, C. Dégut, A. Trinquier, M. Bourguet, P. Barraud, S. Cianferani, B. F. Luisi, C. Condon*, C. Tisné*, Structures of B. subtilis maturation RNases captured on 50S ribosome with pre-rRNAs, Mol Cell 80 (2020), p. 227-236, doi: 10.1016/j.molcel.2020.09.008
P. Barraud*, A. Gato, M. Heiss, M. Catala, S. Kellner & C. Tisné*, Time-resolved NMR monitoring of RNA maturation, Nature Communications 10 (2019), p. 3373-3387. doi: 10.1038/s41467-019-11356-w
C. Dégut#, M. Roovers#, P. Barraud, F. Brachet, A. Feller, V. Larue, A. Al Refaii, J. Caillet, L. Droogmans*, C. Tisné*, Structural characterisation of B. subtilis m1A22 tRNA methyltransferase TrmK: Insights into tRNA recognition, Nucleic Acids Res. 47 (2019), p. 4736-4750. doi: 10.1093/nar/gkz230
We settled within the UMR 8261 in October 2016 to develop a structural biology team focused on the study of RNA. Our competencies are focused on the biochemistry of protein and RNA as well as on structural biology and biophysics (NMR, X-ray crystallography, modeling, calorimetry, fluorescence). We use and develop different methods of structural biology, including NMR spectroscopy, to study the biogenesis, architecture and interactions of RNAs with various partners, thus allowing them to perform their functions. Indeed, RNAs are major players in many biological processes which often include one or more essential steps in which RNA undergoes structural rearrangements involving, for example, cleavage of an intron, the incorporation of post-transcriptionnal modification or more globally modification of the secondary or tertiary interactions upon binding of a ligand (small molecule, protein or another RNA). These structural changes will eventually act on cell function. The study of these structural changes motivates our work. The projects we are developing mainly concern the study of RNA chaperones and the study of the maturation of RNAs (tRNA and rRNA). Our projects benefit from the recent installation in June 2016 of a Bruker 700 MHz NMR spectrometer (Equipex CACSICE) and a cryoprobe on this spectrometer since January 2018.
RMN Plateform of IBPC
(Scientific director: P. Barraud)