Présentation de l'EGM
Description: L'unité «Expression Génétique Microbienne» (EGM; UMR8261 CNRS, Université Paris Cité), a été fondé par Marianne Grunberg-Manago au début des années 60. Elle développe des projets de recherche fondamentaux sur l’expression génétique des micro-organismes (E. coli, B. subtilis, Synechocystis et S. cerevisiae) par des approches génétiques, biochimiques et structurales. L’ARN, sous toutes ses formes, joue un rôle central dans nos travaux, qui concernent les étapes clés dans le contrôle de l’expression génétique : la transcription des gènes, la stabilité des ARNm et leur traductibilité. L'unité compte actuellement 35 personnes réparties en 5 équipes scientifiquement autonomes.
1. Maturation et dégradation de l'ARN (Dirigée par Ciarán Condon)
2. Biogénèse, architecture et interactions des ARNs (Dirigée par Carine Tisné)
3. Contrôle de l'expression génétique par les ARNs (Dirigée par Eliane Hajnsdorf et Maude Guillier)
4. Physiologie et régulation de la synthèse protéique (Dirigée par Grégory Boël)
5. Contrôle transcriptionnel et post-transcriptionnel de l'expression génétique (Dirigée par Harald Putzer)
L'EGM souhaite une bonne retraite à Kyle Tanner qui dirigeait l'équipe Hélicases à ARN.
Prochains Séminaires EGMVendredi 26 Avril (11h30)
Nicolas KINT
«Flagellum assembly in Caulobacter crescentus is governed by multiple molecular mechanisms»
(Sorbonne Université)
Actualités de l'EGM 
Team Recruitment
The Microbial Gene Expression unit (EGM/UMR8261 CNRS Université Paris Cité) is seeking to recruit a dynamic new group or new group leader broadly interested in the field of RNA biology in bacteria, archaea, yeast or organelles (e.g. mitochondria). Candidates will be expected to apply for their own funding (ANR, ATIP, ERC). About 40 m2 of equipped laboratory space will be available at the Institut de Biologie Physico-Chimique (IBPC) in Paris, starting from the spring of 2023. The new group will also have access to the crystallography, NMR, mass-spectrometry and functional genomics platforms, in addition to the shared equipment (Imager, ultracentrifuges, microscopes, qRT-PCR …) of the IBPC. Candidates who do not already have a permanent position will be expected to apply to the CNRS to stabilize their employment situation.
The 6 research groups of the EGM currently use structural, biochemical and genetic approaches to study RNA maturation, modification, and post-transcriptional regulation of translation and mRNA decay. We are looking to expand on these areas of interest and on the experimental approaches used.
Candidates should send a CV and brief project proposal (<5 pages), including a rationale for joining the EGM, to ciaran.condon@ibpc.fr
Récentes Publications EGM
Yeter-Alat H., Belgareh-Touzé N., Huvelle E., Banroques J. and Tanner K. (2023) The DEAD-Box RNA Helicase Ded1 Is Associated with Translating Ribosomes, Genes 14(8), 1566. https://doi.org/10.3390/genes14081566
Marcos-Viquez J, Rodríguez-Hernández A, Álvarez-Añorve LI, Medina-García A, Plumbridge J, Calcagno ML, Rodríguez-Romero A, Bustos-Jaimes I. (2023) Substrate binding in the allosteric site mimics homotropic cooperativity in the SIS-fold glucosamine-6-phosphate deaminases. Protein Sci. Jun;32(6):e4651. doi: 10.1002/pro.4651.
Allouche D, Kostova G, Hamon M, Marchand CH, Caron M, Belhocine S, Christol N, Charteau V, Condon C, Durand S. (2023) New RoxS sRNA Targets Identified in Bacillus subtilis by Pulsed SILAC. Microbiol Spectr. 2023 Jun 20:e0047123. doi: 10.1128/spectrum.00471-23.
Deves V, Trinquier A, Gilet L, Alharake J, Condon C, Braun F. (2023) Shutdown of multidrug transporter bmrCD mRNA expression mediated by the ribosome-associated endoribonuclease (Rae1) cleavage in a new cryptic ORF RNA. 29(8):1108-1116. doi: 10.1261/rna.079692.123.
Trinquier A, Condon C, Braun F. (2023) Effect of tRNA Maturase Depletion on Levels and Stabilities of Ribosome Assembly Cofactor and Other mRNAs in Bacillus subtilis Microbiol Spectr. 11(2):e0513422. doi: 10.1128/spectrum.05134-22
P. Barraud , C. Tisné (2023). Cracking the case of m7G modification in human tRNAs, Nat Struct Mol Biol doi: 10.1038/s41594-023-00937-5
Jagodnik J., Tjaden B., Ross W. and Gourse R.L. (2023) “Identification and characterization of RNA binding sites for (p)ppGpp using RNA-DRaCALA” Nucleic Acids Res. 51(2):852-869. https://doi.org/10.1093/nar/gkac1224
Fostier CR, Ousalem F, Leroy EC, Ngo S, Soufari H, Innis CA, Hashem Y, Boël G. (2023) “Regulation of the macrolide resistance ABC-F translation factor MsrD.” Nat Commun. 14(1):3891. doi: 10.1038/s41467-023-39553-8.
Ousalem F, Singh S, Bailey NA, Wong KH, Zhu L, Neky MJ, Sibindi C, Fei J, Gonzalez RL, Boël G, Hunt JF. Comparative genetic, biochemical, and biophysical analyses of the four E. coli ABCF paralogs support distinct functions related to mRNA translation (2023) bioRxiv. doi: 10.1101/2023.06.11.543863. Preprint.
Korobeinikova A, Laalami S, Berthy C, Putzer H. (2023) “RNase Y Autoregulates Its Synthesis in Bacillus subtilis.” Microorganisms. 11(6):1374. doi: 10.3390/microorganisms11061374.
Wiegard JC, Damm K, Lechner M, Thölken C, Ngo S, Putzer H, Hartmann RK (2023) Processing and decay of 6S-1 and 6S-2 RNAs in Bacillus subtilis. RNA. doi: 10.1261/rna.079666.123.
Bronchain O, Ducos B, Putzer H, Delagrange M, Laalami S, Philippe-Caraty L, Saroul K, Ciapa B (2023) Presenilin natural antisense transcription in the sea urchin: a general control of gene expression during development? J Cell Sci. doi: 10.1242/jcs.261284
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