Les Publications

Publications antérieures à 2019 –>
Autres publications (hors UMR7141) des  membres du laboratoire –>

2020

Caspari O D
Introduction of a leaky stop codon as molecular tool in Chlamydomonas reinhardtii - 2020 - PLoS ONE, 15(8): e0237405 - https://doi.org/10.1371/journal.pone.0237405
Expression of proteins in the chloroplast or mitochondria of the model green alga Chlamydomonas reinhardtii can be achieved by directly inserting transgenes into organellar genomes, or through nuclear expression and post-translational import. A number of tools have been developed in the literature for achieving high...

Manfellotto F, Stella GR, Falciatore A, Brunet C, Ferrante MI
Engineering the Unicellular Alga Phaeodactylum tricornutum for Enhancing Carotenoid Production - 2020 - Antioxidants, 9(8):757 - https://doi.org/10.3390/antiox9080757
Microalgae represent a promising resource for the production of beneficial natural compounds due to their richness in secondary metabolites and easy cultivation. Carotenoids feature among distinctive compounds of many microalgae, including diatoms, which owe their golden color to the xanthophyll fucoxanthin...

Garrido C, Caspari O D, Choquet Y, Wollman F-A and Lafontaine I
Evidence Supporting an Antimicrobial Origin of Targeting Peptides to Endosymbiotic Organelles - 2020 - Cells, 9(8),1795 -
Mitochondria and chloroplasts emerged from primary endosymbiosis. Most proteins of the endosymbiont were subsequently expressed in the nucleo-cytosol of the host and organelle-targeted via the acquisition of N-terminal presequences, whose evolutionary origin remains enigmatic. Using a quantitative assessment of their physico-chemical properties,...

Beauzamy L, Delacotte J, Bailleul B, Tanaka K, Nakanishi S, Wollman F-A, Lemaître F
Mediator-Microorganism Interaction in Microbial Solar Cell: a Fluo-Electrochemical Insight - 2020 - Anal Chem, 92(11):7532-7539 - https://doi.org/10.1021/acs.analchem.9b05808
Microbial solar cells that mainly rely on the use of photosynthesic organisms are a promising alternative to photovoltaics for solar electricity production. In that way, we propose a new approach...

Boussac A, Sellés J, Sugiura M
What can we still learn from the electrochromic band-shifts in Photosystem II? - 2020 - BBA, 1861(5–6):148176 - https://doi.org/10.1016/j.bbabio.2020.148176
Electrochromic band-shifts have been investigated in Photosystem II (PSII) from Thermosynechoccocus elongatus. Firstly, by using Mn-depleted PsbA1-PSII and PsbA3-PSII in which the QX absorption of PheD1 differs, a band-shift in the QX region of PheD2...

Lavoie M, Saint-Béat B, Strauss J, Guérin S, Allard A, Hardy S V, Falciatore A and Lavaud J
Genome-Scale Metabolic Reconstruction and in Silico Perturbation Analysis of the Polar Diatom Fragilariopsis cylindrus Predicts High Metabolic Robustness - 2020 - Biology, 9(2):30 - https://www.mdpi.com/2079-7737/9/2/30
Diatoms are major primary producers in polar environments where they can actively grow under extremely variable conditions. Integrative modeling using a genome-scale model (GSM) is a powerful approach to decipher the complex interactions between components of diatom...

Ozawa S, Cavaiuolo M, Jarrige D, Kuras R, Rutgers M, Eberhard S, Drapier D, Wollman F-A, Choquet Y
The OPR Protein MTHI1 Controls the Expression of Two Different Subunits of ATP Synthase CFo in Chlamydomonas reinhardtii - 2020 - Plant Cell, 32(4):1179-1203 - https://doi.org/10.1105/tpc.19.00770
In Chlamydomonas reinhardtii, chloroplast gene expression is tightly regulated post-transcriptionally by gene-specific trans-acting protein factors. Here we report the identification of an OctotricoPeptide Repeat (OPR) protein, MDH1, critical for the biogenesis of chloroplast ATP synthase CF0....

Falciatore A, Jaubert M, Bouly JP, Bailleul B, Mock T
Diatom Molecular Research Comes of Age: Model Species for Studying Phytoplankton Biology and Diversity - 2020 - Plant Cell, 32(3):547-572 - https://doi.org/10.1105/tpc.19.00158
Diatoms are the most diverse group of algae with at least 100,000 species. Contributing approximately 20% of annual global carbon fixation, they underpin major aquatic food webs and drive global biogeochemical cycles...

Bujaldon S, Kodama N, Rathod M K, Tourasse N, Ozawa S-I, Sellés J, Vallon O, Takahashi Y, Wollman F-A
The BF4 and p71 antenna mutants from Chlamydomonas reinhardtii - 2020 - Biochim Biophys Acta, 1861(4):148085 - https://doi.org/10.1016/j.bbabio.2019.148085
Two pale green mutants of the green alga Chlamydomonas reinhardtii, which have been used over the years in many photosynthesis studies, the BF4 and p71 mutants, were characterized and their mutated gene identified in the nuclear genome...

Gilet J, Conte R, Torchet C, Benard L and Lafontaine I
Additional layer of regulation via convergent gene orientation in yeasts - 2020 - Mol Biol Evol, 37(2):365-378 - https://doi.org/10.1093/molbev/msz221
Convergent gene pairs can produce transcripts with complementary sequences. We had shown that mRNA duplexes form in vivo in Saccharomyces cerevisiae via interactions of mRNA overlapping 3’-ends and can lead to post-transcriptional regulatory events. ...

2019

Dow L, Stock F, Peltekis A, Szamosvári D, Prothiwa M, Lapointe A, Böttcher T, Bailleul B, Vyverman W, Kroth PG, Lepetit B
The multifaceted inhibitory effects of an alkyl quinolone on the diatom Phaeodactylum tricornutum - 2019 - Chembiochem 21(8):1206-1216 - https://doi.org/10.1002/cbic.201900612
The mechanisms underlying interactions between diatoms and bacteria are crucial to understand diatom behaviour and proliferation, and can result in far-reaching ecological consequences. Recently, 2-alkyl-4-quinolones have been isolated...

Theis J, Lang J, Spaniol B, Ferté S, Niemeyer J, Sommer F K, Zimmer D, Venn B, Mehr S F, Mühlhaus T, Wollman F-A, Schroda M
The Chlamydomonas deg1c mutant accumulates proteins involved in high light acclimation - 2019 - Plant Physiol, 181(4):1480-1497 - https://doi.org/10.1104/pp.19.01052
Degradation of periplasmic proteins (Deg)/high temperature requirement A (HtrA) proteases are ATP-independent serine endopeptidases that perform key aspects of protein quality control in all domains of life. Here, we characterized Chlamydomonas reinhardtii DEG1C, ...

Viola S, Bailleul B, Yu J, Nixon P, Sellés J, Joliot P and Wollman F-A
Probing the electric field across thylakoid membranes in cyanobacteria - 2019 - PNAS, 116(43):21900-21906 - https://doi.org/10.1073/pnas.1913099116
In plants, algae, and some photosynthetic bacteria, the ElectroChromic Shift (ECS) of photosynthetic pigments, which senses the electric field across photosynthetic membranes, is widely used to quantify the activity of the photosynthetic chain. In cyanobacteria, ECS signals...

Buck JM, Sherman J, Bártulos CR, Serif M, Halder M, Henkel J, Falciatore A, Lavaud J, Gorbunov MY, Kroth PG, Falkowski PG, Lepetit B.
Lhcx proteins provide photoprotection via thermal dissipation of absorbed light in the diatom Phaeodactylum tricornutum. - 2019 - Nat Commun, 10(1):4167 - https://doi.org/10.1038/s41467-019-12043-6
Diatoms possess an impressive capacity for rapidly inducible thermal dissipation of excess absorbed energy (qE), provided by the xanthophyll diatoxanthin and Lhcx proteins. By knocking out the Lhcx1 and Lhcx2 genes...

Majeran W, Wostrikoff K, Wollman F-A and Vallon O
Role of ClpP in the Biogenesis and Degradation of RuBisCO and ATP Synthase in Chlamydomonas reinhardtii - 2019 - Plants, 8(7) - http://dx.doi.org/10.3390/plants8070191
Ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO) associates a chloroplast- and a nucleus-encoded subunit (LSU and SSU). It constitutes the major entry point of inorganic carbon into the biosphere as it catalyzes photosynthetic CO2 fixation...

Bowler C. and Falciatore A
Phaeodactylum tricornutum - 2019 - Trends in Genetics. 35(9):706-707. - https://doi.org/10.1016/j.tig.2019.05.007
Diatoms, key photosynthetic micro-eukaryotes, found in all aquatic ecosystems are classified based on their symmetry into pennates (elongated) and centrics (radial symmetry). Phaeodactylum tricornutum is a marine pennate species that has become an experimental model used to understand diatom biology...

Annunziata R, Ritter A, Fortunato A E, Manzotti A, Cheminant-Navarro S, Agier N, Huysman M J J, Wing P, Bones A, Bouget F-Y, Cosentino Lagomarsino M, Bouly J-P and Falciatore A
bHLH-PAS protein RITMO1 regulates diel biological rhythms in the marine diatom Phaeodactylum tricornutum - 2019 - PNAS, 116(26):13137-13142 - https://doi.org/10.1073/pnas.1819660116
Periodic light–dark cycles govern the timing of basic biological processes in organisms inhabiting land as well as the sea...

Eberhard S, Valuchova S, Ravat J, Fulnev cek J, Jolivet P, Bujaldon S, Lemaire S D, Wollman F-A, Teixeira M T, Riha K and Xu Z
Molecular characterization of Chlamydomonas reinhardtii telomeres and telomerase mutants - 2019 - Life Science Alliance, 2(3) - http://doi.org/10.26508/lsa.201900315
Telomeres are repeated sequences found at the end of the linear chromosomes of most eukaryotes and are required for chromosome integrity. Expression of the reverse-transcriptase telomerase allows for extension of telomeric repeats to counteract natural telomere shortening...

Nawrocki W, Bailleul B, Cardol P, Rappaport F, Wollman F-A and Joliot P
Maximal cyclic electron flow rate is independent of PGRL1 in Chlamydomonas - 2019 - Biochim Biophys Acta, 1860(5):425-432 - https://doi.org/10.1016/j.bbabio.2019.01.004
Cyclic electron flow (CEF) is defined as a return of the reductants from the acceptor side of Photosystem I (PSI) to the pool of its donors via the cytochrome b6f. It is described to be complementary to the linear electron flow and essential for photosynthesis...

Rohr M, Ries F, Herkt C, Gotsmann VL, Westrich LD, Gries K, Trösch R, Christmann J, Chaux F, Jung M, Zimmer D, Mühlhaus T, Sommer FK, Schroda M, Keller S, Möhlmann T, Willmund F
The role of plastidic trigger factor serving protein biogenesis in green algae and land plants - 2019 - Plant Physiol, 179:1093-1100 - https://doi.org/10.1104/pp.18.01252
Biochemical processes in chloroplasts are important for virtually all life forms. Tight regulation of protein homeostasis and the coordinated...

Viola S, Cavaiuolo M, Drapier D, Eberhard S, Vallon O, Wollman F-A and Choquet Y
MDA1, a nucleus‐encoded factor involved in the stabilization and processing of the atpA transcript in the chloroplast of Chlamydomonas - 2019 - Plant J, 98(6):1033–1047 - https://doi.org/10.1111/tpj.14300
In Chlamydomonas reinhardtii, chloroplast gene expression is tightly regulated post‐transcriptionally by gene‐specific trans‐acting protein factors. Here, we report the molecular identification of an OctotricoPeptide Repeat (OPR) protein,...

Jolivet P, Serhal K, Graf M, Eberhard S, Xu Z, Luke B and Teixeira M T
A subtelomeric region affects telomerase-negative replicative senescence in Saccharomyces cerevisiae - 2019 - Scientific Reports, 9(1):1845 - https://doi.org/10.1038/s41598-018-38000-9
In eukaryotes, telomeres determine cell proliferation potential by triggering replicative senescence in the absence of telomerase. In Saccharomyces cerevisiae, senescence is mainly dictated by the first telomere that reaches a critically short length, activating a DNA-damage-like response...

Takegawa Y, Nakamura M, Nakamura S, Noguchi T, Sellés J, Rutherford A W, Boussac A and Sugiura M
New insights on ChlD1 function in Photosystem II from site-directed mutants of D1/T179 in Thermosynechococcus elongatus - 2019 - Biochim Biophys Acta, 1860(4):297-309 - https://doi.org/10.1016/j.bbabio.2019.01.008
The monomeric chlorophyll, ChlD1, which is located between the PD1PD2 chlorophyll pair and the pheophytin, PheoD1, is the longest wavelength chlorophyll in the heart of Photosystem II and is thought to be the primary electron donor. Its central Mg2+ is liganded to a water molecule...

Stock F, Syrpas M, Graff van Creveld S, Backx S, Blommaert L, Dow L, Stock W, Ruysbergh E, Lepetit B, Bailleul B, Sabbe K, De Kimpe N, Willems A, Kroth P G, Vardi A, Vyverman W and Mangelinckx S
N-Acyl Homoserine Lactone Derived Tetramic Acids Impair Photosynthesis in Phaeodactylum tricornutum - 2019 - ACS Chem. Biol, 14(2):198-203 - https://doi.org/10.1021/acschembio.8b01101
Marine bacteria contribute substantially to nutrient cycling in the oceans and can engage in close interactions with microalgae. Many microalgae...

De Mia M, Lemaire S D, Choquet Y and Wollman F-A
Nitric Oxide Remodels the Photosynthetic Apparatus upon S-Starvation in Chlamydomonas reinhardtii - 2019 - Plant Physiol, 179(2):718-731 - https://doi.org/10.1104/pp.18.01164
Many photosynthetic autotrophs have evolved responses that adjust their metabolism to limitations in nutrient availability. Here we report a detailed characterization of the remodeling of photosynthesis upon sulfur starvation under heterotrophy and photo-autotrophy...

Nawrocki W, Bailleul B, Picot D, Cardol P, Rappaport F, Wollman F-A and Joliot P
The mechanism of cyclic electron flow - 2019 - Biochim Biophys Acta 1860(5):433-438 - https://doi.org/10.1016/j.bbabio.2018.12.005
Apart from the canonical light-driven linear electron flow (LEF) from water to CO2, numerous regulatory and alternative electron transfer pathways exist in chloroplasts. One of them is the cyclic electron flow around Photosystem I (CEF), contributing to photoprotection of both Photosystem I and II (PSI, PSII) and supplying extra ATP to fix atmospheric carbon....

Nawrocki W J, Buchert F, Joliot P, Rappaport F, Bailleul B and Wollman F-A
Chlororespiration controls growth under intermittent light - 2019 - Plant Physiol, 179(2):630-639 - https://doi.org/10.1104/pp.18.01213
Whereas photosynthetic function under steady-state light conditions has been well characterized, little is known about its changes that occur in response to light fluctuations. Chlororespiration, a simplified respiratory chain, is widespread across all photosynthetic lineages, but its role remains elusive. Here, we show that chlororespiration plays a crucial role in intermittentlight conditions...