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Our lab is interested in identifying new ribonucleases in B. subtilis and how they are regulated. We recently identified and characterized the new RNase Rae1/YacP which binds to the A site of the ribosome to cleave its RNAs targets. We also study the mechanisms of action of some of newly-identified RNA processing enzymes at the atomic level. Structural studies on the B. subtilis pyrophosphohydrolase, BsRppH, for example, led to the discovery that this enzyme primarily deprotects mRNAs with a G-residue in the second position. [More information…]


We are now interested in how the activity of these enzymes can be modulated by small regulatory RNAs. We have shown that the RoxS sRNA, which is also found in the Gram-positive pathogen Staphylococcus aureus, is implicated in the oxidative stress response and controls the NAD/NADH ratio in B. subtilis. RoxS can modulate the degradation of mRNAs targets either positively or negatively. [More information…]





News




New publications:



  1. Oerum, S., Dendooven, T., Catala, M., Gilet, L., Dégut, C., Trinquier, A., Bourguet, M., Barraud, P., Cianferani, S., Ben F. Luisi, B. F.., Condon, C.* and C. Tisné*. (2020). Structures of B. subtilis maturation RNases captured on 50S ribosome with pre-rRNAs. Mol Cell. https://doi.org/10.1016/j.molcel.2020.09.008

  2. Pellegrini, O., Gilet, L., Trinquier, A., Tolcan, A., Allouche, D., Durand, S., Braun, F. and C. Condon (2020). Assay of Bacillus subtilis ribonuclease activity in vitro. In: Methods in Molecular Biology: RNA remodeling proteins, Methods and Protocols. Ed: Marc Boudvillain. Springer 2nd Edition. In press

  3. Gilet, L., Pellegrini, O., Trinquier, A., Tolcan, A., Allouche, D., Braun, F., Durand, S. and C. Condon (2020). Assay of Bacillus subtilis ribonuclease activity in vivo. In: Methods in Molecular Biology: RNA remodeling proteins, Methods and Protocols. Ed: Marc Boudvillain. Springer 2nd Edition. In press

  4. Cai, Y., Usher, B., Gutierrez, C., Tolcan, A., Mansour, M., Fineran, P., Condon, C., Neyrolles, O., Genevaux, P. and T. R. Blower (2020). A nucleotidyltransferase toxin inhibits growth of Mycobacterium tuberculosis through inactivation of tRNA acceptor stems.Science Adv 6:eabb6651 DOI: 10.1126/sciadv.abb6651.

  5. Šiková, M., Wiedermannová, J., Převorovský, M., Barvík, I., Sudzinová, P., Kofroňová, O., Benada, O., Šanderová, H., Condon, C., and L. Krásný (2020). The Torpedo Effect in Bacillus subtilis: RNase J1 Resolves Stalled Transcription Complexes. EMBO J. 39:e102500. https://doi.org/10.15252/embj.2019102500



Congratulations to Anastasia Tolcan who defended her PhD Monday November 30th, 2020

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