Vie de l'IBPC
Willy Bienvenut - Understanding chloroplast protein N-terminal acetylation: what can we learn from large-scale N-terminomics?
Understanding chloroplast protein N-terminal acetylation:
what can we learn from large-scale N-terminomics?
I2BC - Institut de Biologie Intégrative de la Cellule
Université Paris Saclay
invité par Stéphane Lemaire et Francis-André Wollman
le lundi 17 décembre 2018 à 11h - Salle de conférence de l'IBPC - 3e étage
N-terminal acetylation and associated modifications are major events determining protein fate and half-life. One of the most frequent cytosolic co-translational modifications is N-terminal acetylation (NTA). This modification is also known to occur post-translationally after the excision of the chloroplastic transit peptide (TP). Although few tools are currently available to predict mature protein N-terminus (e.g. NetAcet or Terminator3) or TP length (e.g. TargetP/ChloroP, TPpred), they are not always accurate. The eNergiome project was focused on the characterization of mature A. thaliana and H. sapiens N-termini, and especially mitochondrial and plastidic proteins that are encoded by the nuclear genome. Our large-scale N-terminomics analyses provide more than 200,000 experimental N-termini related to 6,000 distinct proteins. Curated experimental data were collected in eNergiomeDB and used for the development of new prediction tools (the N-TerPred tool suite) which outperform the existing tools especially for mitochondrial/chloroplast TP predictions. Furthermore, the collected data improve our knowledge of chloroplast NTA that triggered investigations to identify and characterize the dedicated N-acetyltransferases.