Vie de l'IBPC
Séminaires
Séminaire "MEMBRANES & MOLECULES"
31/05/2016
Marion Hamon et Christophe Marchand - Quantitative mass spectrometry: Labeling, or not labeling, that is the question!
Mardi 31 mai 2016, 11h30
Marion Hamon et Christophe Marchand
Laboratoire de Biologie Moléculaire et Cellulaires des Eucaryotes, UMR8226
IBPC, 13 rue Pierre et Marie Curie, 75005 Paris
Quantitative mass spectrometry:
Labeling, or not labeling, that is the question!
Mass spectrometry (MS) is an old but powerful analytical tool. Since the discovery, at the end of the latest century, of the MALDI and Electrospray ionization modes, MS is able to analyze large biomolecules (e.g. proteins and nucleic acids). The latest technical improvements render MS able to deal with complex samples such as proteomes; and nowadays, for sequenced organisms, thousands of proteins can be routinely identified in a reasonable time.
If identification is a prerequisite, quantification is often needed to understand the spatio-temporal aspects of biological processes. In proteomics, several strategies involving metabolic/chemical labeling or not (label-free) have been developed to face this challenge. We will present these different strategies with a particular emphasis on two strategies under development in the IBPC Proteomics Platform. The first strategy, involving Isotope Coded Affinity Tag labeling, allowed us to identify in C. reinhardtii around 600 proteins interacting with the small cytosolic oxidoreductase thioredoxin (Trx) but also to propose more than 1000 putative Trx-targeted cysteines. The second one consists in an Extracted Ion Chromatogram-based label free approach which can be applied to any type of biological samples by using the free Maxquant software. We will present our first results obtained with this approach using synthetic samples containing defined amount of human proteins spiked in S. cerevisiae soluble protein extracts.
Institut de Biologie Physico-Chimique
13, rue Pierre et Marie Curie
75005 PARIS
3ème étage, Salle de Conférences
Invités par Bruno Miroux