UMR8261 Expression Génétique Microbienne

CNRS / Université Paris Diderot Paris 7

Directeur : Harald Putzer, Directeur-adjoint : Ciarán Condon

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BIOGENESIS, ARCHITECTURE AND INTERACTIONS OF RNAs

Resp : Carine Tisné

Phone : 01 58 41 50 13

   We settled within the UMR 8261 in October 2016 to develop a structural biology team focused on the study of RNA. Our competencies are focused on the biochemistry of protein and RNA as well as on structural biology and biophysics (NMR, X-ray crystallography, modeling, calorimetry, fluorescence). We use and develop different methods of structural biology, including NMR spectroscopy, to study the biogenesis, architecture and interactions of RNAs with various partners, thus allowing them to perform their functions. Indeed, RNAs are major players in many biological processes which often include one or more essential steps in which RNA undergoes structural rearrangements involving, for example, cleavage of an intron, the incorporation of post-transcriptionnal modification or more globally modification of the secondary or tertiary interactions upon binding of a ligand (small molecule, protein or another RNA). These structural changes will eventually act on cell function. The study of these structural changes motivates our work. The projects we are developing mainly concern the study of RNA chaperones and the study of the maturation of RNAs (tRNA and rRNA). Our projects benefit from the recent installation in June 2016 of a Bruker 700 MHz NMR spectrometer (Équipex CACSICE) and will benefit from the next installation of a cryoprobe on this spectrometer.

 

 

 

The Team

     
Carine Tisné DR2 CNRS
Pierre Barraud CR2 CNRS
Marjorie Catala IE2 CNRS
Stephanie Ørum Post-Doc
Assia Mouhand Ph. D. Student
Allegra Mboukou
Ph. D Student
Alexandre Gato
IE CDD

 

 

 

Publications

  1. I. Beusch, P. Barraud*, A. Moursy, A. Cléry, F.H.-T. Allain*. Tandem hnRNP A1 RNA recognition motifs act in concert to repress the splicing of survival motor neuron exon 7. eLife (2017) e25736.

  2. Oerum S., Dégut C., Barraud P., Tisné C. "m1A Post-Transcriptional Modification in tRNAs" Biomolecules 7 (2017) E20.

  3. H. Guyon, F. Mavré, M. Catala, S. Turcaud, F. Brachet, B. Limoges*, C. Tisné* & L. Micouin*, Use of Redox Probe for Electrochemical RNA-ligand Binding Assay in Microliter Droplets,
    Chem Comm 53 (2017), 1140-1143.

  4. C. Dégut, P. Barraud*, V. Larue, C. Tisné*, Backbone resonance assignments of the m1A22 tRNA methyltransferase TrmK from Bacillus subtilis.
    Biomolecular NMR assignment (2016) 10 (2016), p. 253-257.

  5. A. Belfetmi, L. Zargarian, C. Tisné, D. Sleiman, N. Morellet, E. Lescop, O. Maskri, B. René, Y. Mély, P. Fossé, O. Mauffret, Insights into the mechanisms of destabilization of RNA secondary structure by the HIV-1 nucleocapsid protein.
    RNA 22 (2016), p. 506-517.

  6. C. Dégut, L. Ponchon, M. Folly-Klan, P. Barraud & C. Tisné*, The m1A58 modification in eubacterial tRNA: an overview of tRNA recognition and mechanism of catalysis by TrmI.
    Biophys. Chemistry 210 (2016), 27-34.

  7. C. Dégut, A. Monod, F. Brachet, C. Crépin & C. Tisné*, In vitro/in vivo production of tRNA for X-ray studies.
    Methods Mol Biol 1320 (2016), p. 37-57.

  8. L. Iannazzo, E. Benedetti, M. Catala, M. Ethève-Quelquejeu, C. Tisné, L. Micouin. Monitoring of reversible broronic acid-diol interactions by fluorine NMR spectroscopy in aqueous media.
    Org. Biomol. Chem. 13 (2015), p. 8817-8821.

  9. M. E. Khouri, M. Catala, B. Seijo, J. Chabal, C. Tisné, F. Dardel & Ponchon L., Expression and purification of RNA-Protein complexes in Escherichia coli.
    Methods Mol Biol. 1316 (2015), p. 25-31.

  10. A. Blond, E. Ennifar*, C. Tisné* & L. Micouin*, The design of RNA binders : targeting the HIV replication cycle as a case study.
    ChemMedChem 9 (2014), p. 1982-1996.

  11. M. Mori, A. Nucci, M. Chiara Dasso Lang, N. Humbert, C. Boudier, F. Debaene, S. Sanglier-Cianferani, M. Catala, P. Schult-Dietrich, U. Dietrich, C. Tisné, Y. Mély & M. Botta, Functional and Structural Characterization of a 2-amino-4-phenylthiazole inhibitor of the HIV-1 Nucleocapsid Protein with Antiviral Activity.
    ACS Chem Biol 9 (2014), p. 1950-1955

  12. L. Zargarian, C. Tisné, P. Barraud, N. Morellet, B. René, Y. Mély, P. Fossé & O. Mauffret, The distinct roles of the two zinc fingers of the HIV-1 nucleocapsid protein could be modulated by its linker residues.
    PloS ONE 9 (2014), e102150.

  13. D. Sleiman, S. Bernacchi, S. X. Guerrero, F. Brachet, V. Larue, J.-C. Paillart* & C. Tisné*, Characterization of RNA binding and chaperoning activities of HIV-1 Vif protein: importance of the C-terminal unstructured tail.
    RNA Biol 11 (2014), p. 906-920.

  14. L. Ponchon, M. Catala, B. Seijo, M. El Khouri, F. Dardel, S. Nonin-Lecomte & C. Tisné, Co-expression of RNA/protein complexes in Escherichia coli and applications to RNA biology.
    Nucleic Acids Res, 41 (2013), e150.

  15. D. Sleiman, P. Barraud, F. Brachet & C. Tisné*, The interaction between tRNALys3 and the primer activation signal deciphered by NMR spectroscopy.
    PLoS ONE, 8 (2013): e64700.

  16. J. Piton, V. Larue, Y. Thillier, A. Dorléans, O. Pellegrini, I. Li de la Sierra-Gallay, J.J. Vasseur, F. Debart, C. Tisné & C. Condon, The B. subtilis RNA deprotection enzyme RppH recognizes guanosine in the second position of its substrates.
    PNAS, 110 (2013), p. 8858–8863.

  17. T. Lombès, R. Moumné, V. Larue, E. Prost, M. Catala, T. Lecourt, F. Dardel, L. Micouin* & C. Tisné*, Investigation of RNA-ligand interaction by 19F NMR spectroscopy using fluorinated probes.
    Angewandte Chemie Int. Ed. Engl. 51 (2012), p. 9530-9534 .

  18. D. Sleiman, V. Goldschmidt, P. Barraud, R. Marquet, J.-C. Paillart & C. Tisné*, Initiation of HIV-1 reverse transcription and functional role of nucleocapsid-mediated tRNA/viral genome interactions.
    Virus Research 169 (2012), p. 324-339.

  19. J. Batisse, S. Guerrero, S. Bernacchi, D. Sleiman, C. Gabus, J.-L. Darlix, R. Marquet, C. Tisné, J.-C. Paillart, The role of Vif oligomerization and RNA chaperone activity in HIV-1 replication.
    Virus Research 169 (2012), p. 361-376.

  20. R. Moumné, M. Catala, V. Larue, L. Micouin & C. Tisné*, Fragment-based design of small RNA binders: promising developments and contribution of NMR.
    Biochimie 94 (2012), p. 1607-1619.

  21. A. Guelorget, P. Barraud, C. Tisné & B. Golinelli-Pimpaneau, Structural comparison of tRNA m1A58 methyltransferases revealed different molecular strategies to maintain their oligomeric architecture under extreme conditions.
    BMC Structural Biology 11 (2011), p. 48.

  22. R. Moumné, M. Pasco, E. Prost, T. Lecourt, L. Micouin* & C. Tisné*, Fluorinated diaminocyclopentanes as chiral sensitive probes of RNA structure.
    J. Am. Chem. Soc. 132 (2010), p. 13111-13113.

  23. R. Moumné, V. Larue, B. Seijo, T. Lecourt, L. Micouin* & C. Tisné*, Tether influence on the binding properties of tRNALys3 ligands designed by a fragment based approach.
    Org. Biomol. Chem. 8 (2010), p. 1154-1159.

  24. F Chung, C. Tisné*, T. Lecourt, B. Seijo, F. Dardel & L. Micouin*, Design of tRNALys3 ligands: fragment evolution and linker selection guided by NMR spectroscopy.
    Chemistry, a European journal 15 (2009), p. 7109-7116.

  25. V. Larue, B. Seijo, C. Tisné & F. Dardel, 1H, 13C and 15N NMR assignments of the E. coli peptide deformylase in complex with a natural inhibitor called actinonin.
    Biomolecular NMR assignments 3 (2009), p. 153-155.

  26. P. Barraud, E. Schmitt, Y. Mechulam, F. Dardel & C. Tisné*, A unique conformation of the anticodon stem-loop is associated with the capacity of tRNAfMet to initiate protein synthesis.
    Nucl. Acids Res., 36 (2008), p. 4894-4901.

  27. P. Barraud, J.C. Paillart, R. Marquet & C. Tisné*, Advances in the structural understanding of Vif proteins.
    Curr. HIV Res., 6 (2) (2008), p. 91-99.

  28. P. Barraud, B. Golinelli-Pimpaneau, C. Atmanene, S. Sanglier, A. Van Dorsselauer, L. Droogmans, F. Dardel & C. Tisné*, Crystal structure of Thermus thermophilus tRNA m1A58 methyltransferase and biophysical characterization of its interaction with tRNA.
    J. Mol. Biol., 377 (2008), p. 535-550.

  29. P. Barraud, C. Gaudin, F. Dardel & C. Tisné*, New insights into the formation of HIV-1 reverse transcription initiation complex.
    Biochimie, 89 (2007), p. 1204-1210.

  30. P. Barraud, F. Dardel & C. Tisné*, Optimizing HSQC experiment for the observation of exchange broadened signals in RNA-protein complexes.
    C R Chimie, 11 (2008), p. 91-99.

  31. F Chung, C. Tisné*, T. Lecourt, F. Dardel* & L. Micouin*, NMR-guided fragment-based approach for the design of tRNALys3 ligands.
    Angewandte Chemie Int. Ed. Engl., 46(2007), p. 4489-4491.

  32. C. Tisné*, F. Guillière & F. Dardel, NMR-based identification of peptides that specifically recognize the D-arm of tRNA.
    Biochimie, 87(2005), p. 885-888.

  33. C. Tisné*, Structural bases of the annealing of HIV-1 reverse transcription primer tRNALys3 to the viral RNA.
    Curr. HIV Res., 3 (2)(2005), p. 147-156.

  34. C. Tisné, B. P. Roques & F. Dardel, The annealing mechanism of HIV-1 reverse transcription primer onto the viral genome.
    J. Biol. Chem., 279 (2004), p. 3588-3595.

  35. C. Gaudin, S. Nonin-lecomte, C. Tisné, S. Corvaisier, V. Bordeau, F. Dardel & B. Felden, The tRNA-like domains of E. coli and A. aeolicus transfer messenger RNA : Structural and functional studies.
    J. Mol. Biol. 331(2003), p. 457-471.

  36. C. Tisné*, B. P. Roques & F. Dardel, Specific recognition of primer tRNALys3 by HIV-1 nucleocapsid protein: involvement of the zinc fingers and of the N-terminal basic extension.
    Biochimie, 85 (2003), p. 557-561.

  37. C. Tisné & F. Dardel, Optimisation of a peptide library for screening specific RNA ligands by flow-injection NMR.
    Comb. Chem. High Troughput Screen., 5(2002), p513-518.

  38. C. Tisné*, B. P. Roques & F. Dardel*, Heteronuclear NMR studies of the interaction of tRNALys3 with HIV-1 nucleocapsid protein.
    J. Mol. Biol., 306 (2001), p. 443-454.

  39. B. Brutscher, J. Boisbouvier, E. Kupce, C. Tisné, F. Dardel, D. Marion & J.-P. Simorre, Base-type-selective high resolution 13C edited NOESY for sequential assignment of large RNA.
    J. Biomol. NMR., 19 (2001), p. 141-151.

  40. C. Tisné, M. Rigourd, R. Marquet, C. Ehresmann & F. Dardel, NMR and biochemical characterisation of recombinant human tRNALys3 expressed in E. coli: Identification of post-transcriptional nucleotide modifications required for efficient initiation of HIV-1 reverse transcription.
    RNA, 6 (2000), p. 1403-1412.

  41. C. Tisné*, M. Delepierre & B. Hartmann*, How NF-?B can be attracted by its cognate DNA.
    J. Mol. Biol., 293 (1999), p. 139-150.

  42. C. Tisné, B. Hartmann & M. Delepierre, NF-?B binding mechanism: an NMR and modelling study of a GGG->CTC mutation.
    Biochemistry, 38 (1999), p. 3883-3894.

  43. C. Tisné*, E. Hantz, B. Hartmann & M. Delepierre*, Solution structure of a non-palindromic 16 base-pair DNA related to the HIV-1 ?B site: evidences for BI-BII equilibrium inducing a global dynamic curvature of the duplex.
    J. Mol. Biol., 279 (1998), p. 127-142.

  44. C. Tisné*, C. Simenel, E. Hantz & M. Delepierre, 31P NMR conformational studies of non-palindromic DNA duplexes related to HIV-1 enhancer.
    J. Chim. Phys., 2 (1998), p. 412-417.

  45. V. Larue, J. Gharbi-benarous, F. Acher, F. Arulmozhi, C. Tisné, N. Todeschi, R. Azerad & J.-P. Girault, Solution conformation of ?, ? or ? methylglutamyl-containing derivatives as probes of vitamin K-dependent carboxylase using molecular modelling and nuclear magnetic resonance.
    Int. J. Biol. Macromol., 20 (1997), p. 131-159.

  46. C. Tisné, C. Simenel, E. Hantz, F. Schaeffer & M. Delepierre, Backbone conformational study of a non-palindromic 16 base-pair DNA duplex exploring 2D 31P-1H heteronuclear inverse spectroscopy: Assignment of all NMR phosphorus resonances and measurement of 3J 31P-1H3' coupling constants.
    Magn. Reson. Chem., 34 (1996), p. s115-s124.

 

Les quatre thésards de l'équipe ("cherchez l'intrus")

 

 

Former members

Hélène Guyon thèse soutenue le 24 octobre 2016 ("Mise au point d'une méthode de mesure d'interaction ligand-ADN par éléctrochimie")
Paul Caillat

Stagiaire M2 BGA (UPMC)

janvier à juin 2017

     

 

 

 

Recruitment

contact us

 

 

 

Pictures

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"1 an déjà"

 

"colloque RMN-IDF 25 avril 2017, IBPC"

 

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17/05/2017 et ça bosse !

 

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fin du "déménagement" : (23 septembre 2016)

 

 

 

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