UMR8261 Expression Génétique Microbienne

CNRS / Université Paris Diderot Paris 7

Directeur : Harald Putzer, Directeur-adjoint : Ciarán Condon

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RNA control of gene expression

 

Resp : Eliane Hajnsdorf

 

  Bacteria have to respond and adapt very rapidly to environmental stress or changes in growth conditions. One of the strategies they have developed to do so is to regulate gene expression at the post-transcriptional level, using RNA and proteins as regulators. Our research interests are (1) to gain insights into the mechanisms by which gene expression in E. coli is modulated, directly or indirectly, by molecules as diverse as small regulatory RNAs (sRNAs) and proteins involved in RNA metabolism such as ribonucleases, poly(A)polymerase, Hfq and possibly others, and (2) to dissect the links between different regulatory networks involving sRNAs and other transcriptional or translational regulators.

  Our team was created in September 2012 with the fusion of two previous teams  working in the field of post-transcriptional regulation of gene expression in Gram-negative bacteria, with a special emphasis put on the role of RNA in this process (« Translational control of gene expression in Gram negative bacteria » directed by M. Springer and « Genetic expression and RNA stability » directed by P. Regnier and E. Hajnsdorf).

 

 

 

 

 

 

 

 

 

 


 

RNA based mechanisms of gene expression

 

  Gene expression plays a key role in allowing bacteria to adapt to their environment. While transcription was the first step of gene expression to be shown as subject to regulation, later studies demonstrated that post-transcriptional control exists as well. Furthermore, it has been known for decades that RNA can play a crucial role in regulation of gene expression in bacteria and other kingdoms of life. This has been confirmed those last fifteen years as we have seen an explosion both in the number of regulatory RNAs identified and in our understanding of the variety of mechanims used by these molecules to control gene expression.

  Our studies range from the role of cis-elements of the target-mRNA in translational control by ribosomal proteins to the mechanisms of control exerted by cis- or trans-encoded antisense RNAs or the role of other actors, such as ribonucleases or the RNA chaperone Hfq.

  We are working with Escherichia coli, which is a model organism of choice for these studies given the knowledge that we have of this bacterium and the available tools to study it. But a lot of our findings are expected to be applicable to other pathogenic or non-pathogenic enterobacteria.

 

 

 

 

 

 

 

 

 

 


 

   

 

 

RNA maturation and stability

  In E. coli, RNA stability depends on endoribonucleolytic cleavages which initiate the process of degradation but also on the addition of poly(A) tails which facilitate the elimination of RNA fragments by exoribonucleases. Other actors, such as RNA helicases, ribosomes, Hfq protein and regulatory RNAs also participate in these processes.

  In spite of the fact that most (if not all) ribonucleases that mediate RNA maturation or decay have been identified, their individual functions are not well understood. One of our unexpected findings was that polyadenylation mediates bacterial RNA decay. Long regarded as a characteristic of eukaryotic mRNA, polyadenylation now appears to be a universal process for all classes of RNA. In both types of cells, this degradation pathway plays a role in quality control of RNA by degrading specific non-functional RNA. It also promotes the degradation of small RNA fragments generated by endoribonucleases during maturation or inactivation of the primary transcription allowing their degradation by exoribonucleases. However, the poly(A)-polymerase also modifies the functional stability of mRNA, thus demonstrating that polyadenylation can have a role in E. coli gene expression.

  The Hfq protein is involved in various mechanisms including RNA degradation and translation. This protein has an essential function after stress and participates in the expression of virulence factors in many pathogenic bacteria. It may act either directly on mRNA or indirectly by facilitating the interaction of mRNA with sRNAs. Interestingly, Hfq also modifies the abundance of certain mRNAs independently of their degradation. Moreover it has a role in the poly(A)dependent degradation pathway. We use in vivo and in vitro approaches to analyze the mode of action of Hfq and its function in cell metabolism.

  Our main objective is to understand how these various factors participate in RNA based regulation of gene expression.

 

 

 

 

 

 

 

 

 

 


 

 

Small RNAs in cellular networks.

 

  Numerous studies point to a strong connection between sRNAs and other regulators. Indeed, not only is the synthesis of sRNAs highly regulated at the transcriptional level by transcription factors or alternative sigma factors, but many sRNAs also directly regulate synthesis of transcriptional regulators. The biology of the resulting mixed regulatory networks is still poorly understood. We are studying several examples that point out to a previously unsuspected complexity. For instance, two unrelated small RNAs that regulate synthesis of the PhoQ/PhoP two-component system by a similar mechanism have completely different effects on expression of the PhoP regulon. Together with the known competition between sRNAs for Hfq or between multiple targets of a given sRNA, our results highligt the requirement for systems biology when studying sRNA-mediated regulations.

 

 

 

 

 

 

 

 

 

 

 


 

 

Current Lab members

 

     
Eliane Hajnsdorf-Casnabet DR1 CNRS
Joël Caillet CR1 CNRS
Claude Chiaruttini CR1 CNRS
Maude Guillier CR1 CNRS
Fanette Fontaine MC P7
  Thao Nguyen Le Lam Post-Doc ANR
  Mark Rutgers Post-Doc Labex
Jonathan Jagodnik Ph. D
Anaîs Brosse AI
Maxence Lejars Ph.D Student

 

Former Lab members

Equipe "Contrôle de l'expression génétique par les ARN"

  • Alexandre Maes Post-Doc (janvier 2014- septembre 2015)
  • Céline Gracia IE2 (déc. 2009-oct. 2016)
  • Nathalie Sisattana CDD IE (Octobre 2015-Juin 2016)
  • Rachid Boudjelloul CDD AI (Juin 2013-Juillet 2014)
  • Harry Kemble M2 (2013)
  • Rudi Zimmermann 2ème année AgroParisTech (Avril-Août 2017)
  • Agata Staszak ERASMUS (2013)
  • Fanny Vazzoler M1 (Février-Avril 2014)
  • Victoria Prudent  M1 (juin-juillet 2014)

Equipe « Expression génétique et stabilité de l’ARN »

  • Philippe Régnier PRCE1 Paris 7 (Emérite depuis septembre 2013 UPR9080)
  • Véronique Arluison MC2 Paris 7 (2001- 2008)
    Anne Vanet MC1 Paris 7
  • Marie Dancer-Thibonnier Post-doc ATER Paris 7 (décembre 2010- août 2011)
  • Florent Busi Post-doc ATER Paris 7 (octobre 2006- septembre 2008)
  • Jacques Le Derout AI CNRS (départ octobre 2009)
  • Mathieu Ballouche PhD
    Alexandre Maes PhD (diplôme en 2010)
    Marc Folichon PhD (diplôme en 2005)
    Paulo Marujo PhD (diplôme en 2004)
    Jeanette Haugel-Nielsen PhD (diplôme en 1998)
    Frédérique Braun PhD (diplôme en 1997)
  • Elise Gasiorowski M2 (2013)
    Olatz Ruiz-Larabeiti Programme Leonardo da Vinci (2011)
    Jessica Berlier ERASMUS (2010)
    Pau Packard ERASMUS (2009)
    Farouk Satouri (juin-juillet 2008)
    Géraldine Joanny ERASMUS (2005)
    Elisa Gaetani ERASMUS (2004)
    Katarzyna Ziolkowska ERASMUS (2003)
  • Maxence Lejars M1 (mai-juin 2015)
    Jean - Michel Desfontaines Magistère (juillet-septembre 2012)
    Romain Bouvier BTS (mai-juin 2011)
    Diane Lebrun L3 ENS Lyon (2010)

Visitors

  • Irina Boni 2 mois chaque année (2002-2010)
    Marc Uzan DR2 CNRS (avril 2013-décembre 2013)

Tteam « Contrôle traductionnel chez les bactéries »

  • Mathias Springer DR1 CNRS Emérite dans l’équipe Condon depuis septembre 2012
  • Frédéric Allemand IR2 CNRS
    Anna Korobeinikova Post-doc ANR
    Alexei Korepanov Post-doc Marie Curie
    Pascale Aliprandi Post-doc ANR
  • Audrey Coornaert PhD (diplôme en 2012)
  • Anthony Calvino M2 (2011)
    Francisco Martins Programme Leonardo da Vinci (2011)

 

 

Main publications

    2017

  1. Jagodnik, J., Chiaruttini, C. Guillier, M.. (2017). "Stem-Loop Structures within mRNA Coding Sequences Activate Translation Initiation and Mediate Control by Small Regulatory RNAs." Mol Cell 68(1): 158-170 e153.

  2. Maes A., Gracia C. , Innocenti N. , Zang K. , Aurell E. & Hajnsdorf E. (2017) "Landscape of RNA polyadenylation in E. coli" Nucleic Acids Res. 45, 2746-2756, https://doi.org/10.1093/nar/gkw894

  3. Jagodnik, J., Brosse, A., Le Lam, T.N., Chiaruttini, C., Guillier, M. (2017) "Mechanistic study of base-pairing small regulatory RNAs in bacteria". Methods 117: 67-76 doi: 10.1016/j.ymeth.2016.09.012

  4. Le Lam, T.N., Morvan, C., Liu, W., Bohn, C., Jaszczyszyn, Y. & Bouloc, P., (2017) Finding sRNA-associated phenotypes by competition assays: An example with Staphylococcus aureus". Methods 117: 21-27 doi: 10.1016/j.ymeth.2016.11.018

  5. 2016

  6. Jagodnik, J., Thieffry, D. and Guillier, M. (2016) Bacterial Small RNAs in Mixed Regulatory Circuits, in Stress and Environmental Regulation of Gene Expression and Adaptation in Bacteria (ed F. J. de Bruijn), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9781119004813.ch33

  7. Brosse, A.; Korobeinikova, A.; Gottesman, S.; Guillier, M. (2016) "Unexpected properties of sRNA promoters allow feedback control via regulation of a two-component system" Nucl. Acids Res. 2016 44: 9650-9666

  8. Ruiz-Larrabeiti, O., Hernández, A., Gracia, C., Sevillano, E., Gallego, L., Hajnsdorf, E. & Kaberdin, V. (2016) "A new custom microarray for sRNA profiling in Escherichia coli" FEMS Microbiology Letters, 363, (13). (Editor's choice)

  9. Fontaine, F., Gasiorowski, E., Gracia, C., Ballouche, M., Caillet, J., Marchais, A. & Hajnsdorf, E. "The small RNA SraG participates in PNPase homeostasis." RNA 22, 1560-73

  10.  

    2014

  11. Boudry, P., Gracia, C., Monot, M., Caillet, J., Saujet L., Hajnsdorf, E., Dupuy, B., Martin-Verstraete, I.  & Soutourina, O.  (2014) "Pleiotropic role of the RNA chaperone protein Hfq in the human pathogen Clostridium difficile" J. Bacteriol., 196, 3234-32-48

  12. Caillet J., Gracia C., Fontaine, F. and Hajnsdorf, E.(2014) "Clostridium difficile Hfq can replace Escherichia coli Hfq for most of its function" RNA ; 20: 1567-1578

  13. Hammann P., Parmentier D., Cerciat M., Reimegard J., Helfer AC., Boisset S., Guillier M., Vandenesch F., Wagner EG., Romby P. and Fechter P., (2014), A method to map changes in bacterial surface composition induced by regulatory RNAs in Escherichia coli and Staphylococcus aureus, Biochimie, 2014 Nov;106:175-9. doi: 10.1016/j.biochi.2014.07.011..

  14. Wenner, N., Maes, A., Cotado-Sampayo, M.  & Lapouge, K. (2014) NrsZ: a novel, processed, nitrogen-dependent, small non-coding RNA that regulates Pseudomonas aeruginosa PAO1 virulence. Environ Microbiol. 16, 1053-68

  15. 2013

  16.  Bos J., Duverger Y., Thouvenot B., Chiaruttini C., Branlant C., Springer M., Charpentier B. & Barras F. (2013) The sRNA RyhB regulates the synthesis of the Escherichia coli methionine sulfoxide reductase MsrB but not MsrA, PLoS One, 8(5): e63647.

  17. Maes, A., Gracia,C.,  Bréchemier-Baey, D., Hamman, P., Chatre, E., Lemelle, L., Bertin, P. N. & Hajnsdorf, E. (2013) Role of polyadenylation in regulation of the flagella cascade and motility in E. coli. Biochimie, 95, 410-418.

  18. Régnier, Ph. &  Hajnsdorf,  E. (2013) The interplay of Hfq, poly(A) polymerase I and exoribonucleases at the 3' ends of RNAs resulting from Rho-independent termination: a tentative model. RNA Biology 10(4)

  19. Mandin P. and Guillier M. (2013). Expanding control in bacteria: interplay between small RNAs and transcriptional regulators to control gene expression, Current Opinion in Microbiology, 16(2): 125-32.

  20. Coornaert A., Chiaruttini C., Springer M. & Guillier M.(201) Post-transcriptional control of the Escherichia coli PhoQ-PhoP two-component system by multiple sRNAs involves a novel pairing region of GcvB. PLoS Genet, 2013;9(1):e1003156. doi: 10.1371/journal.pgen.1003156. Epub 2013 Jan 3.

    2012

  21. Hajnsdorf, E. & Boni, I. V. (2012) Multiple activities of RNA-binding proteins S1 and Hfq. Biochimie 94, 1544-1553.

  22. Maes, A., Gracia, C., Hajnsdorf, E. & Régnier, Ph. (2012) Search for poly(A) polymerase targets in E. coli reveals its implication in surveillance of Glu tRNA processing and degradation of stable RNAs. Mol Microbiol, 83, 436-451.

  23. Thomason, M. K., Fontaine, F., De Lay, N. & Storz, G. (2012) A small RNA that regulates motility and biofilm formation in response to changes in nutrient availability in Escherichia coli. Mol Microbiol, 84, 17-35.

  24. Ni, M., Decrulle, A. L., Fontaine, F., Demarez, A., Taddei, F. & Lindner, A. B. (2012) Pre-disposition and epigenetics govern variation in bacterial survival upon stress. PLoS Genet. 8(12),

    2011

  25. Mangeol, P., Bizebard, T., Chiaruttini, C., Dreyfus, M., Springer, M., & Bockelmann, U. (2011) Probing ribosomal protein–RNA interactions with an external force. Proc. Natl. Acad. Sci. U.S.A. 108, 18272-18276.

  26. 2010

  27. Busi, F., Le Dérout, J., Cerciat, M., Régnier, Ph. & Hajnsdorf, E.(2010) Is the secondary putative RNA-RNA interaction site relevant to GcvB mediated regulation of oppA mRNA in Escherichia coli ? Biochimie 92, 1458-1461.

  28. Coornaert, A., Lu. A., Mandin, P., Springer, M., Gottesman, S. & Guillier, M. (2010) MicA sRNA links the PhoP regulon to cell envelope stress. Mol. Microbiol., 76, 467-479.

  29. Le Dérout, J., Boni, I. V., Régnier, Ph. & Hajnsdorf, E. (2010) Hfq affects mRNA levels independently of degradation. BMC Mol. Biol. 11, 17.

  30. Noinaj, N., Guillier, M., Barnard, T. J. & Buchanan, S. K. (2010). TonB-dependent transporters: regulation, structure, and function. Annu Rev Microbiol 64, 43-60. http://www.ncbi.nlm.nih.gov/pubmed/20420522

  31. 2009

  32. Andrade, J. M., Hajnsdorf, E., Régnier, Ph. & Arraiano, C. (2009) The Poly(A)-dependent degradation pathway of rpsO mRNA is primarily mediated by RNase R. RNA 15, 316-326.

  33. Chiaruttini, C., Allemand, F. & Springer, M. (2009) Structural probing of RNA thermosensors. Methods Mol. Biol. 540, 233-245. http://www.ncbi.nlm.nih.gov/pubmed/19381564

  34. Timsit, Y., Acosta, Z., Allemand, F., Chiaruttini, C. & Springer, M. (2009) The role of disordered ribosomal protein extensions in the early steps of eubacterial 50 s ribosomal subunit assembly. Int. J. Mol. Sci. 10, 817-834.

  35. Régnier, Ph. & Hajnsdorf, E. (2009) Poly(A)-assisted RNA decay and modulators of RNA stability. In : Progress in Nucleic Acids Research and Translational Science 85, 137-185.

  36. 2008

  37. Haentjens-Sitri, J., Allemand, F., Springer, M. & Chiaruttini, C. (2008) A competition mechanism regulates the translation of the Escherichia coli operon encoding ribosomal proteins L35 and L20. J. Mol. Biol. 375, 612-625.

  38. Reichenbach, B., Maes, A., Kalamorz, F., Hajnsdorf, E. & Görke, B. (2008) The small RNA GlmY acts upstream of the sRNA GlmZ in the activation of glmS expression and is subject to regulation by polyadenylation in Escherichia coli. Nucleic Acids Res. 36, 2570–2580.

  39. Roovers, M., Oudjama, Y., Kaminska, K. H., Purta, E., Caillet, J., Droogmans, L. & Bujnicki, J. M. (2008) Sequence-structure-function analysis of the bifunctional enzyme MnmC that catalyses the last two steps in the biosynthesis of hypermodified nucleoside mnm5s2U in tRNA. Proteins 71, 2076-2085.

  40. Guillier, M. & Gottesman, S. (2008). The 5' end of two redundant sRNAs is involved in the regulation of multiple targets, including their own regulator. Nucleic Acids Res 36, 6781-6794.

  41. 2007

  42. Allemand, F., Haentjens, J., Chiaruttini, C., Royer, C.& Springer, M. (2007) Escherichia coli ribosomal protein L20 binds as a single monomer to its own mRNA bearing two potential binding sites. Nucleic Acids Res. 35, 3016-3031.

  43. Caillet, J., Graffe, M., Eyermann, F., Romby, P. & Springer, M. (2007) Mutations in residues involved in zinc binding in the catalytic site of Escherichia coli threonyl-tRNA synthetase confer a dominant lethal phenotype. J. Bacteriol. 189, 6839-6848.

  44. Joanny, G., Le Derout, J., Bréchemier-Baey, D., Labas, V., Vinh, J., Régnier, Ph. & Hajnsdorf, E. (2007) Polyadenylation of a functional mRNA controls gene expression in E. coli. Nucleic Acids Res. 35, 2494-2502.

  45. 2006

  46. Ziolkowska, K., Derreumaux, Ph., Folichon, M., Pellegrini, O., Régnier, Ph., Boni, I. & Hajnsdorf, E. (2006) Hfq variant with altered RNA binding functions. Nucleic Acids Res. 34, 709-720.

  47. Timsit, Y., Allemand, F., Chiaruttini, C. & Springer, M. (2006). Coexistence of two protein folding states in the crystal structure of ribosomal protein L20. EMBO Rep 7, 1013-1018.

  48. Guillier, M., Gottesman, S. & Storz, G. (2006). Modulating the outer membrane with small RNAs. Genes Dev 20, 2338-2348.

  49. Tjaden, B., Goodwin, S. S., Opdyke, J. A., Guillier, M., Fu, D. X., Gottesman, S. & Storz, G. (2006). Target prediction for small, noncoding RNAs in bacteria. Nucleic Acids Res 34, 2791-2802.

  50. Gottesman, S., McCullen, C. A., Guillier, M., Vanderpool, C. K., Majdalani, N., Benhammou, J., Thompson, K. M., FitzGerald, P. C., Sowa, N. A. & FitzGerald, D. J. (2006). Small RNA regulators and the bacterial response to stress. Cold Spring Harb Symp Quant Biol 71, 1-11.

  51. Guillier, M. & Gottesman, S. (2006). Remodelling of the Escherichia coli outer membrane by two small regulatory RNAs. Mol Microbiol 59, 231-247.

  52. 2005

  53. Folichon, M., Allemand, F., Régnier, Ph. & Hajnsdorf, E. (2005) Stimulation of poly(A) synthesis by E.coli poly(A) polymerase I is correlated with Hfq binding to poly(A) tails. FEBS J. 272, 454-463.

  54. Guillier, M., Allemand, F., Graffe, M., Raibaud, S., Dardel, F., Springer, M. & Chiaruttini, C. (2005). The N-terminal extension of Escherichia coli ribosomal protein L20 is important for ribosome assembly, but dispensable for translational feedback control. RNA 11, 728-738.

  55. Guillier, M., Allemand, F., Dardel, F., Royer, C. A.,Springer, M. & Chiaruttini, C. (2005). Double molecular mimicry in Escherichia coli: binding of ribosomal protein L20 to its two sites in mRNA is similar to its binding to 23S rRNA. Mol Microbiol 56, 1441-1456.

  56. 2004

  57. Marujo, P.E., Braun, F., Haugel-Nielsen, J., Le Derout, J., Arraiano, C.M. and Regnier, P. (2003) Inactivation of the decay pathway initiated at an internal site by RNase E promotes poly(A)-dependent degradation of the rpsO mRNA in Escherichia coli. Mol Microbiol. 50, 1283-1294.

    2003

  58. Folichon, M., Arluison, V., Pellegrini, O., Huntzinger, E., Régnier, Ph. & Hajnsdorf, E. (2003) The poly(A) binding protein Hfq protects RNA from RNase E and exoribonucleolytic degradation. Nucleic Acids Res. 31, 7302-7310.

  59. Le Derout, J., Folichon, M., Briani, F., Dehò, G., Régnier, P. & Hajnsdorf, E. (2003) Hfq affects the length and the frequency of short oligo(A) tails at the 3’ end of Escherichia coli rpsO mRNAs Nucleic Acids Res. 31, 4017-4023.

  60. Raibaud, S., Vachette, P., Guillier, M., Allemand, Chiaruttini, C., & Dardel, F. (2003). How bacterial ribosomal protein L20 assembles with 23S ribosomal RNA and its own messenger RNA. J. Biol. Chem. 278, 36522-30.

  61. 2002

  62. Arluison, V., Derreumaux, Ph., Allemand, F., Folichon, M., Hajnsdorf, E. & Régnier, P. (2002) Structural modeling of the Sm-like protein HFq from E. coli. J. Mol. Biol., 320, 705-712.

  63. Raibaud, S., Lebars, I., Guillier, M., Chiaruttini, C., Bontems, F., Rak, A., Garber, M., Allemand, F., Springer, M. & Dardel, F. (2002). NMR structure of bacterial ribosomal protein L20: implications for ribosome assembly and translational control. J. Mol Biol., 323:143-51.

  64. Johansson, J., Mandin, P., Renzoni, A., Chiaruttini, C., Springer, M. & Cossart, P. (2002). An RNA thermosensor controls expression of virulence genes in Listeria monocytogenes. Cell, 110:551-61.

  65. Guillier, M., Allemand, F., Raibaud, S., Dardel, F., Springer, M. & Chiaruttini, C. (2002). Translational feedback regulation of the gene for L35 in Escherichia coli requires binding of ribosomal protein L20 to two sites in its leader mRNA: a possible case of ribosomal RNA-messenger RNA molecular mimicry. RNA, 8:878-89.

  66. 2000

  67. Hajnsdorf, E. & Régnier, P.(2000) Host factor HFq (HF-1) of Escherichia coli stimulates elongation of poly(A) tails by poly(A) polymerase I. Proc. Natl Acad. Sc. USA, 97, 1501-1505.

  68. Marujo, P., Hajnsdorf, E., Le Derout, J., Andrade, R., Arraiano, C. M. & Régnier, P.(2000) RNase II prevents synthesis of oligo(A)tails which destabilize the rpsO mRNA. RNA 6, 1079-90

Collaborations

 
  • Erik Aurell AlbaNova University Center, Stockholm, Suède.
  • Frederic Barras CNRS UMR, LCB, Marseille
  • Ulrich Bockelmann CNRS UMR 7083, ESCPI, Paris
  • Irina Boni Institute of Bioorganic Chemistry, Moscow, Russia.
  • Louis Droogmans ULB Bruxelles.
  • Daniel Gautheret UMR 8621 CNRS, IGM, Orsay.
  • Susan. Gottesman National Cancer Institute, Bethesda, USA
  • Vladimir Kaberdin University of the Basque Country, Leioa, Espagne.
  • C. Tisné et S. Nonin-Lecomte UMR8015 CNRS, Fac. de Pharmacie, Paris.
  • I. Verstraete et O. Soutourina Institut Pasteur, Paris.

Funding since 2004

  • 2015-2017 : ANR Programme Jeunes Chercheurs. UnifyRNA Coordinateur M. Guillier "Propriétés uniques des petits ARN régulateurs dans les réseaux transcriptionnels et le contrôle traductionnel"
  • CNRS, Université Paris 7 Denis Diderot et LABEX Dynamo (2012-2020)
  • 2013-2017 : ANR Programme Blanc asSUPYCO. Coordinateur I. Iost, partenaires C. Condon et E. Hajnsdorf.
  • 2011-2014 : ANR Programme Jeunes Chercheurs. Coordinateur M. Guillier « Cascades de régulation chez Escherichia coli: le rôle des petits ARN régulateurs et des systèmes à deux composants dans la régulation de la composition membranaire et de l'homéostasie du magnésium».
  • 2005-2008 : ANR Programme Blanc. Coordinateur : Mathias Springer « Le rôle des protéines intrinsèquement dépliées dans l’assemblage du ribosome » 
  • 2011 : Union Européenne, programme Leonardo da Vinci. E. Hajnsdorf
  • 2011 : Union Européenne, programme Leonardo da Vinci. M. Guillier
  • 2007-2010 : Programme Actions Universitaires Intégrées Luso-Française (PAUILF)
  • 2005 et 2007/2008 : Ministère des Affaires Etrangères; PAI Galilée, Actions intégrées Luso-Françaises
  • 2014 : Fondation E. de Rothschild
  • 2013-2017 : Labex DYNAMO : Allocation de Recherche
  • 2009-2010 : Fondation pour la Recherche Médicale
  • 2009-2012 : Ministère de l’Enseignement et de la Recherche; Allocation de Recherche sur thème scientifique prioritaire
  • 2005-2009 ; Contrat Quadriennal Ministère de l'Education Nationale
  • 2005 et 2009 : Bonus Qualité Recherche de l’Université Paris 7
  • 2004-2006 : Programme de Recherche Fondamentale en Microbiologie, Maladies Infectieuses et Parasitaires

 

 


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