Institut de Biologie Physico-Chimique

Team members

• Bruno Gasnier, CNRS research director, group leader

• Corinne Sagné,  INSERM scientist

• Christine Anne,  INSERM scientist

• Cécile Debacker, CNRS engineer assistant

• Xiong Chen , Post-Doc

• Raquel Ruivo , PhD Student

• Azita Sharifi , PhD Student

Alumni

Contact : Bruno Gasnier, CNRS UMR 8192, Institut de Biologie Physico-Chimique, 13 rue Pierre et Marie Curie, F-75005 Paris, France; Fax: +33-1-5841 5023 ; E-mail: bruno.gasnier(at)ibpc.fr

Research area

Our group studies the solute compartmentalization of eukaryotic cells and the membrane transporters responsible for this organization, with a special interest for organelles of the endocytic and exocytotic pathways.

We currently focus on two intracellular transport processes :

• the uptake of non-peptide neurotransmitters into synaptic vesicles

• the egress of lysosomal hydrolysis products to the cytosol, a process largely unknown at molecular level.

Selected publications

Rutsch F, Gailus S, Miousse IR, Suormala T, Sagné C, Toliat MR, Nürnberg G,Wittkampf T, Buers I, Sharifi A,
 Stucki M, Becker C, Baumgartner M, Robenek H,Marquardt T, Höhne W, Gasnier B, Rosenblatt DS, Fowler B,
 Nürnberg P.(2009) Identification of a putative lysosomal cobalamin exporter altered in the cblFdefect 
of vitamin B12 metabolism. Nat Genet. Feb;41(2):234-9. PubMed

Sagné C, Gasnier B. (2008) Molecular physiology and pathophysiology of lysosomalmembrane transporters. 
J Inherit Metab Dis. Apr 15. PubMed

Ruivo R, Sharifi A, Boubekeur S, Morin P, Anne C, Debacker C, Graziano JC,Sagné C, Gasnier B. (
2008) Molecular pathogenesis of sialic acid storage diseases:insight gained from four missense mutations
 and a putative polymorphism of human sialin. Biol Cell. Sep;100(9):551-9. PubMed

Gras C, Amilhon B, Lepicard EM, Poirel O, Vinatier J, Herbin M, Dumas S, Tzavara ET, Wade MR, 
Nomikos GG, Hanoun N, Saurini F, Kemel ML, Gasnier B, Giros B, El Mestikawy S. (2008) The vesicular glutamate 
transporter VGLUT3 synergizes striatal acetylcholine tone. Nat Neurosci. Mar;11(3):292-300. PubMed

Aubrey KR, Rossi FM, Ruivo R, Alboni S, Bellenchi GC, Le Goff A, Gasnier B, Supplisson S. (2007 ) The transporters
 GlyT2 and VIAAT cooperate to determine thevesicular glycinergic phenotype. 
J Neurosci. Jun 6;27(23):6273-81. PubMed

Kalatzis, V., Nevo, N., Cherqui, S., Gasnier, B. and Antignac, C. (2004) Molecular pathogenesis of cystinosis: effect of CTNS mutations on the transport activity and subcellular localization of cystinosin. Hum Mol Genet, 13, 1361-1371. PubMed

Gasnier, B. (2004) The SLC32 transporter, a key protein for the synaptic release of inhibitory amino acids. Pflugers Arch, 447, 756-759. PubMed

Boll, M., Daniel, H. and Gasnier, B. (2004) The SLC36 family: proton-coupled transporters for the absorption of selected amino acids from extracellular and intracellular proteolysis. Pflugers Arch, 447, 776-779. PubMed

Gras, C., Herzog, E., Bellenchi, G.C., Bernard, V., Ravassard, P., Pohl, M., Gasnier, B., Giros, B. and El Mestikawy, S. (2002) A third vesicular glutamate transporter expressed by cholinergic and serotoninergic neurons. J Neurosci, 22, 5442-5451. PubMed

Kalatzis, V., Cherqui, S., Antignac, C. and Gasnier, B. (2001) Cystinosin, the protein defective in cystinosis, is a H+-driven lysosomal cystine transporter. EMBO J, 20, 5940-5949. PubMed

Sagné, C., Agulhon, C., Ravassard, P., Darmon, M., Hamon, M., El Mestikawy, S., Gasnier, B. and Giros, B. (2001) Identification and characterization of a lysosomal transporter for small neutral amino acids. Proc Natl Acad Sci U S A, 98, 7206-7211. PubMed

Herzog, E., Bellenchi, G.C., Gras, C., Bernard, V., Ravassard, P., Bedet, C., Gasnier, B., Giros, B. and El Mestikawy, S. (2001) The existence of a second vesicular glutamate transporter specifies subpopulations of glutamatergic neurons. J Neurosci, 21, RC181. PubMed

Bedet, C., Isambert, M.F., Henry, J.P. and Gasnier, B. (2000) Constitutive phosphorylation of the vesicular inhibitory amino acid transporter in rat central nervous system. J Neurochem, 75, 1654-1663. PubMed

Dumoulin, A., Rostaing, P., Bedet, C., Levi, S., Isambert, M.F., Henry, J.P., Triller, A. and Gasnier, B. (1999) Presence of the vesicular inhibitory amino acid transporter in GABAergic and glycinergic synaptic terminal boutons. J Cell Sci, 112, 811-823. PubMed

Sagné, C., El Mestikawy, S., Isambert, M.F., Hamon, M., Henry, J.P., Giros, B. and Gasnier, B. (1997) Cloning of a functional vesicular GABA and glycine transporter by screening of genome databases. FEBS Lett, 417, 177-183. PubMed

Last update: July 13th, 2009