NMR examples from the laboratory:

15N-1H 2D solution-state NMR spectrum of the membrane protein OmpX in solution, in presence of amphipols A8-35 and in H2O (in black) or in D2O (in red). The difference between the two indicates amino acids that are in contact with water.



13C-13C 2D solid-state NMR spectra of the membrane protein MscL in a lipid membrane. On the left, the protein is uniformely labeled with 13C and 15N. On the right, only isoleucines and threonines are enriched in 13C and 15N, using the technique of in vitro synthesis.



Publications




  • L. J. Catoire, M. Damian, F. Giusti, A. Martin, C. van Heijenoort, J.-L. Popot, E. Guittet and J.-L. Banères. Structure of a GPCR Ligand in Its Receptor-Bound State: Leukotriene B4 Adopts a Highly Constrained Conformation When Associated to Human BLT2. J. Am. Chem. Soc. 132:9049-9057 (2010)(Pubmed)PDF

  • A. Abdine, M. A. Verhoeven, K.-H. Park, A. Ghazi, E. Guittet, C. Berrier, C. Van Heijenoort and D. E. Warschawski. Structural study of the membrane protein MscL using cell-free expression and solid-state NMR. J. Magn. Reson. 204:155-159 (2010)(Pubmed)PDF

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