Welcome to the Laboratory of Physical and Chemical Biology of Membrane Proteins

CNRS UMR 7099 - University Paris Diderot - Paris 7

Our laboratory gathers biologists, physicists and chemists who are interested in the structure, structural dynamics and physical chemistry of membrane proteins, either in vivo within a lipid bilayer, or in vitro after solubilization and stabilization by classical or alternative surfactants such as amphipols.

Our proteins of interest cover various domains of fundamental and medical biology:

  • Energy coupling & supramolecular organization of respiratory chain complexes with the structure-function study of the cytochrome b6f complex and the mitochondrial uncoupling proteins.
  • Molecular Signalization Pathway of GPGRs with the study of HEDGEHOG Pathway, the study of Signal Transduction by GPCRs using liquid state NMR and GPCR Cristallization.
  • Transport and Membrane Dynamics in Bacteria including the biochemistry of Iron Transport in Haemophilus, the membrane biogenesis in E. coli, solid state NMR of membranes and membrane proteins and the study of efflux pumps in bacteria.

In addition, all members of the unit contribute to the development of our tool box for membrane proteins studies, which includes: NMR, mass spectrometry, crystallography, physical-chemistry of surfactants, membrane protein production and synthetic biology.


Bruno Miroux

Head of unit

Recently published

A complete and up to date coverage on Membrane Proteins in Aqueous Solutions, Springer International Publisher, 2018, written by Jean-Luc Popot is now available: Web link

A complete and up to date coverage on Membrane Proteins in Aqueous Solutions, Springer International Publisher, 2018, written by Jean-Luc Popot is now available: Web link

M. Casiraghi, E. Point, A. Pozza, K. Moncoq, J.-L. Banères & L.J. Catoire (2019) NMR analysis of GPCR conformational landscapes and dynamics. Mol Cell Endocrinol. 484:69-77. (Pubmed)

S. Petrella, E. Capton, B. Raynal, C. Giffard, A. Thureau, F. Bonneté, P.M. Alzari, A. Aubry & C. Mayer (2019) Overall Structures of Mycobacterium tuberculosis DNA Gyrase Reveal the Role of a Corynebacteriales GyrB-Specific Insert in ATPase Activity. Structure (Pubmed)

Z. Bouhlel, A. A. Arnold, D. E. Warschawski, K. Lemarchand, R. Tremblay and I. Marcotte (2019) Labelling strategy and membrane characterization of marine bacteria Vibrio splendidus by in vivo 2H NMR. Biochim. Biophys. Acta 1861:871-878 (Pubmed)

Membranes & Molecules Seminars

Pierre-Jean Corringer
(Thursday, January 22th 2019, 11h30)
Pasteur Institute, Channel-receptor Unit, CNRS UMR 3571, 25 rue du Docteur Roux, 75015 Paris, France
Allosteric transition of a bacterial pentameric ligand-gated ion channel (abstract)

Workshop

The traditionnal meeting 'ABC of membrane transport' will take place on Friday November 30th 2018 in the Bibliothèque of IBPC, Paris. The final program is being finalized. Confirmed speakers: Sylvie Rebuffat, Dirk-Jan Slotboom, Olivier Fardel.(program).

PhD defense

Federica Angius, October 13th 2017
"Molecular basis of membrane protein production and intracellular membranes proliferation in E. coli"(abstract)

Education

Structural Biochemistry Course IBPC:
From Protein Identification by Mass Spectrometry to Crystal Structure Determination and Molecular Dynamic Analysis.

---From 8th to 16th March 2018---
More details and Program.

Course Integrative Structural Biology​
--Mechanisms of Living Organisms--

---From 16th to 21th July 2018---
More details and Program.

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Last update: 19/02/19

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