Welcome to the Laboratory of Physical and Chemical Biology of Membrane Proteins

CNRS UMR 7099 - University Paris Diderot - Paris 7

Our laboratory gathers biologists, physicists and chemists who are interested in the structure, structural dynamics and physical chemistry of membrane proteins, either in vivo within a lipid bilayer, or in vitro after solubilization and stabilization by classical or alternative surfactants such as amphipols.

Our proteins of interest cover various domains of fundamental and medical biology:

  • Energy coupling & supramolecular organization of respiratory chain complexes with the structure-function study of the cytochrome b6f complex and the mitochondrial uncoupling proteins.
  • Molecular Signalization Pathway of GPGRs with the study of HEDGEHOG Pathway, the study of Signal Transduction by GPCRs using liquid state NMR and GPCR Cristallization.
  • Transport and Membrane Dynamics in Bacteria including the biochemistry of Iron Transport in Haemophilus, the membrane biogenesis in E. coli, solid state NMR of membranes and membrane proteins and the study of efflux pumps in bacteria.

In addition, all members of the unit contribute to the development of our tool box for membrane proteins studies, which includes: NMR, mass spectrometry, crystallography, physical-chemistry of surfactants, membrane protein production and synthetic biology.


Bruno Miroux

Head of unit

Job position

A 2-year post-doc position is available starting from december 2018. Study of the energy landscape of G Protein-Coupled Receptors in lipid discs.
For more information, see http://bit.ly/2OuGZ6M and Post-doc description.

Recently published

A complete and up to date coverage on Membrane Proteins in Aqueous Solutions, Springer International Publisher, 2018, written by Jean-Luc Popot is now available: Web link

D.F. Tifrea, S. Pal, C. Le Bon, F. Giusti, J.-L. Popot, M. J. Cocco, M. Zoonens & L.M. de la Maza(2018) Co-delivery of amphipol-conjugated adjuvant with antigen, and adjuvant combinations, enhance immune protection elicited by a membrane protein-based vaccine against a mucosal challenge with Chlamydia. Vaccine. 36(45):6640-6649 (Pubmed)

R.A. Corey, E. Pyle, W.J. Allen, D.W. Watkins, M. Casiraghi, B. Miroux, I. Arechaga, A. Politis & I. Collinson (2018) Specific cardiolipin-SecY interactions are required for proton-motive force stimulation of protein secretion. PNAS. 115(31):7967-7972 doi:10.1073/pnas.1721536115. (Pubmed)

F. Angius, O. Ilioaia, A. Amrani, A. Suisse, L. Rosset, A. Legrand, A. Abou-Hamdan, M. Uzan, F. Zito & B. Miroux (2018) A novel regulation mechanism of the T7 RNA polymerase based expression system improves overproduction and folding of membrane proteins. Sci Rep. 8(1):8572. (Pubmed)

Membranes & Molecules Seminars

Olivier Lambert
(Wednesday, October 17th 2018, 14h30)
Institut de Recherche en Infectiologie de Montpellier (IRIM), UMR9004-CNRS, université de Montpellier
Solve the” ESKAPE game” to combat antibiotic resistance: CryoEM studies of a tripartite assembly of RND multidrug efflux pumps (abstract)

Workshop

The traditionnal meeting 'ABC of membrane transport' will take place on Friday November 30th 2018 in the Bibliothèque of IBPC, Paris. The final program is being finalized. Confirmed speakers: Sylvie Rebuffat, Dirk-Jan Slotboom, Olivier Fardel.

PhD defense

Federica Angius, October 13th 2017
"Molecular basis of membrane protein production and intracellular membranes proliferation in E. coli"(abstract)

Education

Structural Biochemistry Course IBPC:
From Protein Identification by Mass Spectrometry to Crystal Structure Determination and Molecular Dynamic Analysis.

---From 8th to 16th March 2018---
More details and Program.

Course Integrative Structural Biology​
--Mechanisms of Living Organisms--

---From 16th to 21th July 2018---
More details and Program.

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Last update: 18/10/24

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