2013

C. L. Pocanschi, J.-L. Popot and J. H. Kleinschmidt. Folding and stability of outer membrane protein A (OmpA) from Escherichia coli in an amphipathic polymer, amphipol A8-35. Eur. Biophys. J. 42:103-118 (2013) (Pubmed)
T. Dahmane, F. Rappaport and J.-L. Popot. Amphipol-assisted folding of bacteriorhodopsin in the presence and absence of lipids. Functional consequences. Eur. Biophys. J. 42:85-101 (2013) (Pubmed)
S. Santabarbara, A. P. Casazza, K. Ali, C. K. Economou, T. Wannathong, F. Zito, K. E. Redding, F. Rappaport and S Purton. The requirement for carotenoids in the assembly and function of the photosynthetic complexes in Chlamydomonas reinhardtii. Plant Physiol. 161:535-546 (2013)(Pubmed)

2012

I. Blesneac, S. Ravaud, P. Machillot, M. Zoonens, S. Masscheylen, B. Miroux, M. Vivaudou and E. Pebay-Peyroula. Assaying the proton transport and regulation of UCP1 using solid supported membranes. Eur. Biophys. J. 41:675-679 (2012) (Pubmed)
C. Bechara, G. Bolbach, P. Bazzacco, S. K. Sharma, G. Durand, J.-L. Popot, F. Zito and S. Sagan. MALDI mass spectrometry analysis of membrane protein/amphipol complexes. Anal. Chem. 84:6128-6135 (2012) (Pubmed)

F. Giusti, J.-L. Popot and C. Tribet. Well-defined critical association concentration and rapid adsorption at the air/water interface of a short amphiphilic polymer, amphipol A8-35: a study by Förster resonance energy transfer and dynamic surface tension measurements. Langmuir 28:10372-10380 (2012)(Pubmed)
K. Scheffler, M. Krohn, T. Dunkelmann, J. Jan Stenzel, B. Miroux, S. Ibrahim, O. von Bohlen und Halbach, H.-J. Heinze, L. C. Walker, J. A. Gsponer and J. Pahnke. Mitochondrial DNA polymorphisms specifically modify cerebral b-amyloid proteostasis. Acta Neuropathol. 124:199-208 (2012)(Pubmed)
K. S. Sharma, G. Durand, F. Gabel, P. Bazzacco, C. Le Bon, E. Billon-Denis, L. J. Catoire, J.-L. Popot, C. Ebel and B. Pucci Non-ionic amphiphilic homopolymers: Synthesis, solution properties, and biochemical validation. Langmuir 28:4625-4639 (2012)(Pubmed)
A. Kyrychenko, M. V. Rodnin, M. Vargas-Uribe, S. K. Sharma, G. Durand, B. Pucci, J.-L. Popot and A. S. Ladokhin Folding of diphtheria toxin T-domain in the presence of amphipols and fluorinated surfactants: Toward thermodynamic measurements of membrane protein folding. Biochim. Biophys. Acta 1818:1006-1012 (2012)(Pubmed)
P. Bazzacco, E. Billon-Denis, K. S. Sharma, L. J. Catoire, S. Mary, C. Le Bon, E. Point, J.-L. Banères, G. Durand, F. Zito, B. Pucci, and J.-L. Popot Non-ionic homopolymeric amphipols: Application to membrane protein folding, cell-free synthesis, and solution NMR. Biochemistry, 51:1416-1430 (2012)(Pubmed)
I. Blesneac, S. Ravaud, C. Juillan-Binard, L. A. Barret, M. Zoonens, A. Polidori, B. Miroux, B. Pucci and E. Pebay-Peyroula Production of UCP1 a membrane protein from the inner mitochondrial membrane using the cell free expression system in the presence of a fluorinated surfactant. Biochim. Biophys. Acta 1818:798-805 (2012)(Pubmed)
A. Abdine, K.-H. Park and D. E. Warschawski. Cell-free membrane protein expression for solid-state NMR. Methods Mol. Biol. 831:85-109 (2012)(Pubmed)

2011

T. Althoff, D. J. Mills, J.-L. Popot and W. Kühlbrandt Assembly of electron transport chain components in bovine mitochondrial supercomplex I1III2 IV1. EMBO J. 30:4652-4664 (2011)(Pubmed)
U. Gimsa, E. Kanitz, W. Otten, C. Aheng, M. Tuchscherer, D. Ricquier, B. Miroux and S. M. Ibrahim Alterations in anxiety-like behavior following knockout of the uncoupling protein 2 (ucp2) gene in mice. Life Sci. 89:677-684 (2011)(Pubmed)
T. Dahmane, F. Giusti, L. J. Catoire and J.-L. Popot Sulfonated amphipols: Synthesis, properties and applications. Biopolymers 95:811-823 (2011)(Pubmed)
C. Aheng, N. Ly, M. Kelly, S. Ibrahim, D. Ricquier, M. C. Alves-Guerra and B. Miroux Deletion of UCP2 in iNOS Deficient Mice Reduces the Severity of the Disease during Experimental Autoimmune Encephalomyelitis. PLoS One 6:e22841 (2011)(Pubmed)
J. D. Perlmutter, W. J. Drasler, W. Xie, J. Gao, J.-L. Popot and J. N. Sachs All-atom and coarse-grained molecular dynamics simulations of a membrane protein stabilizing polymer. Langmuir 27:10523-10537 (2011) (Pubmed)
L. J. Catoire, M. Damian, M. Baaden, E. Guittet and J.-L. Banères Electrostatically-driven fast association and perdeuteration allow detection of transferred cross-relaxation for G protein-coupled receptor ligands with equilibrium dissociation constants in the high-to-low nanomolar range. J. Biomol. NMR 50:191-195 (2011)(Pubmed)
J.-L. Banères, J.-L. Popot and B. Mouillac New advances in production and functional folding of G-protein-coupled receptors. Trends Biotechnol. 29:314-322 (2011)(Pubmed)
D. E. Warschawski, A. A. Arnold, M. Beaugrand, A. Gravel, E. Chartrand and I. Marcotte Choosing membrane mimetics for NMR structural studies of transmembrane proteins. Biochim. Biophys. Acta 1808:1957-1974 (2011)(Pubmed)
D. F. Tifrea, G. Sun, S. Pal, G. Zardeneta, M. J. Cocco, J.-L. Popot and L. M. de la Maza Amphipols stabilize the Chlamydia major outer membrane protein and enhance its protective ability as a vaccine. Vaccine 29:4623-4631 (2011)(Pubmed)
J.-L. Popot, T. Althoff, D. Bagnard, J.-L. Banères, P. Bazzacco, E. Billon-Denis, L. J. Catoire, P. Champeil, D. Charvolin, M. J. Cocco, G. Crémel, T. Dahmane, L. M. de la Maza, C. Ebel, F. Gabel, F. Giusti, Y. Gohon, E. Goormaghtigh, E. Guittet, J. H. Kleinschmidt, W. Kühlbrandt, C. Le Bon, K. L. Martinez, M. Picard, B. Pucci, F. Rappaport, J. N. Sachs, C. Tribet, C. van Heijenoort, F. Wien, F. Zito and M. Zoonens Amphipols from A to Z. Annu. Rev. Biophys. 40:379-408 (2011)(Pubmed)
J. Hovers, M. Potschies, A. Polidori, B. Pucci, S. Raynal, F. Bonneté, M. Serrano-Vega, C. Tate, D. Picot, Y. Pierre, J.-L. Popot, R. Nehmé, M. Bidet, I. Mus-Veteau, H. Bußkamp, K.-H. Jung, A. Marx, P. A. Timmins and W. Welte A class of mild surfactants that keep integral membrane proteins water-soluble for functional studies and crystallization. Mol. Memb. Biol. 28:171-181 (2011)(Pubmed)
K.-H. Park, E. Billon-Denis, T. Dahmane, F. Lebaupain, B. Pucci, C. Breyton and F. Zito In the cauldron of cell-free synthesis of membrane proteins: playing with new surfactants. New Biotechnol. 28:255-261 (2011)(Pubmed)
A. Abdine, M. A. Verhoeven and D. E. Warschawski Cell-free expression and labeling strategies for a new decade in solid-state NMR. New Biotechnol. 28:272-276 (2011)(Pubmed)

2010

P. S. Chae, S. G. Rasmussen, R. R. Rana, K. Gotfryd, R. Chandra, M. A. Goren, A. C. Kruse, S. Nurva, C. J. Loland, Y. Pierre, D. Drew, J.-L. Popot, D. Picot, B. G. Fox, L. Guan, U. Gether, B. Byrne, B. Kobilka and S. H. Gellman Maltose-neopentyl glycol (MNG) amphiphiles for solubilization, stabilization and crystallization of membrane proteins. Nat. Methods 7:1003-1008 (2010)(Pubmed)
C. Breyton, B. Pucci and J.-L. Popot. Amphipols and fluorinated surfactants: two alternatives to detergents for studying membrane proteins in vitro. IN HETEROLOGOUS EXPRESSION OF MEMBRANE PROTEINS: METHODS AND PROTOCOLS (MUS-VETEAU, I., ED.). 601:219-245 (2010)(Pubmed)
M. Zoonens and B. Miroux. Expression of Membrane Proteins at the Escherichia coli Membrane for Structural Studies. IN HETEROLOGOUS EXPRESSION OF MEMBRANE PROTEINS: METHODS AND PROTOCOLS (MUS-VETEAU, I., ED.). 601:49-66 (2010)(Pubmed)
A. Abdine, M. A. Verhoeven, K.-H. Park, A. Ghazi, E. Guittet, C. Berrier, C. Van Heijenoort and D. E. Warschawski. Structural study of the membrane protein MscL using cell-free expression and solid-state NMR. J. Magn. Reson. 204:155-159 (2010)(Pubmed)
M. Gueroult, D. Picot, J. Abi-Ghanem, B. Hartmann and M. Baaden. How Cations Can Assist DNase I in DNA Binding and Hydrolysis. PLOS Comput Biol. 6:e1001000 (2010)(Pubmed)
J.-L. Popot. Amphipols, nanodiscs, and fluorinated surfactants: three nonconventional approaches to studying membrane proteins in aqueous solutions. Annu. Rev. Biochem. 79:737-775 (2010)(Pubmed)
L. J. Catoire, M. Damian, F. Giusti, A. Martin, C. van Heijenoort, J.-L. Popot, E. Guittet and J.-L. Banères. Structure of a GPCR Ligand in Its Receptor-Bound State: Leukotriene B4 Adopts a Highly Constrained Conformation When Associated to Human BLT2. J. Am. Chem. Soc. 132:9049-9057 (2010)(Pubmed)
L. J. Catoire, M. Zoonens, C. van Heijenoort, F. Giusti, E. Guittet and J.-L. Popot. Solution NMR mapping of water-accessible residues in the transmembrane beta-barrel of OmpX. Eur. Biophys. J. 39:623-630 (2010)(Pubmed)

2009

A. de Lacroix de Lavalette, L. Barucq, J. Alric, F. Rappaport and F. Zito. Is the redox state of the ci heme of the cytochrome b6f complex dependent on the occupation and structure of the Qi site and vice versa? J. Biol. Chem. 284:20822-20829 (2009)(Pubmed)
A. Pohl, I. Lopez-Montero, F. Rouviere, F. Giusti and P. F. Devaux. Rapid transmembrane diffusion of ceramide and dihydroceramide spin-labelled analogues in the liquid ordered phase. Mol. Membr. Biol. 26:194-204 (2009)(Pubmed)
D. Charvolin, J.-B. Perez, F. Rouviere, F. Giusti, P. Bazzacco, A. Abdine, F. Rappaport, K. L Martinez and J.-L. Popot. The use of amphipols as universal molecular adapters to immobilize membrane proteins onto solid supports. Proc. Natl. Acad. Sci. U. S. A. 106:405-410 (2009)(Pubmed)
L. J. Catoire, M. Zoonens, C. van Heijenoort, F. Giusti, J.-L. Popot and E. Guittet. Inter- and intramolecular contacts in a membrane protein/surfactant complex observed by heteronuclear dipole-to-dipole cross-relaxation. J. Magn. Reson. 197:91-95 (2009)(Pubmed)
M. Laursen, M. Bublitz, K. Moncoq, C. Olesen, J. Vuust Moller, H. S. Young, P. Nissen and J. Preben Morth. Cyclopiazonic acid is complexed to a divalent metal ion when bound to the sarcoplasmic reticulum Ca2+-ATPase. J. Biol Chem. 284:13513-13518 (2009)(Pubmed)
C. Breyton, F. Gabel, M. Abla, Y. Pierre, F. Lebaupain, G. Durand, J.-L. Popot, C. Ebel and B. Pucci. Micellar and biochemical properties of (hemi)fluorinated surfactants are controlled by the size of the polar head. Biophys. J. 97:1077-1086 (2009)(Pubmed)
P. Bazzacco, K.S. Sharma, G. Durand, F. Giusti, C. Ebel, J.-L. Popot and B. Pucci. Trapping and stabilization of integral membrane proteins by hydrophobically grafted glucose-based telomers. Biomacromolecules 10:3317-3326 (2009)(Pubmed)
T. Dahmane, M. Damian, S. Mary, J.-L. Popot and J.-L. Banères. Amphipol-assisted in vitro folding of G protein-coupled receptors. Biochemistry 48:6516-6521 (2009)(Pubmed)
C. Tribet, C. Diab, T. Dahmane, M. Zoonens, J.-L. Popot and F.M. Winnik. Thermodynamic characterization of the exchange of detergents and amphipols at the surfaces of integral membrane proteins. Langmuir 25:12623-12634 (2009)(Pubmed)

2008

M. Zoonens, Y. K. Reshetnyak and D. M. Engelman. Bilayer interactions of pHLIP, a peptide that can deliver drugs and target tumors. Biophys. J. 95:225-235 (2008) (Pubmed)
A. M. S. Duarte, C. J. A. M Wolfs, R. B. M. Koehorst, J.-L. Popot and M. A. Hemminga. Solubilization of V-ATPase transmembrane peptides by amphipol A8-35. J. Pept. Sci. 14:389-393 (2008)(Pubmed)
C. Pecqueur, T. Bui, C. Gelly, J. Hauchard, C. Barbot, F. Bouillaud, D. Ricquier, B. Miroux and C. B. Thompson. Uncoupling protein-2 controls proliferation by promoting fatty acid oxidation and limiting glycolysis-derived pyruvate utilization. FASEB J. 22:9-18 (2008)(Pubmed)
K. Moncoq, G. Kemp, X. Li, L. Fliegel and H. S. Young. Dimeric structure of human Na+/H+ exchanger isoform 1 overproduced in Saccharomyces cerevisiae. J. Biol Chem. 283:4145-4154 (2008)(Pubmed)
K. Shivaji Sharma, G. Durand, F. Giusti, B. Olivier, A.-S. Fabiano, P. Bazzacco, T. Dahmane, C. Ebel, J.-L. Popot and B. Pucci. Glucose-based amphiphilic telomers designed to keep membrane proteins soluble in aqueous solutions: synthesis and physicochemical characterization. Langmuir 24:13581-13590 (2008)(Pubmed)
E. Karatekin, V. S. Tran, S. Huet, I. Fanget, S. Cribier and J.-P. Henry. A 20-nm step toward the cell membrane preceding exocytosis may correspond to docking of tethered granules. Biophys. J. 94:2891-2905 (2008)(Pubmed)
A. de Lacroix de Lavalette, R. P. Barbagallo and F. Zito. Why is it so difficult to construct Q(i) site mutants in Chlamydomonas reinhardtii? C. R. Biol. 331:510-517 (2008)(Pubmed)
A. de Lacroix de Lavalette, G. Finazzi and F. Zito. b6f-Associated chlorophyll: structural and dynamic contribution to the different cytochrome functions. Biochemistry 47:5259-5265 (2008)(Pubmed)
P. Cardol, B. Bailleul, F. Rappaport, E. Derelle, D. Beal, C. Breyton, S. Bailey, F. A. Wollman, A. Grossman, H. Moreau and G. Finazzi. An original adaptation of photosynthesis in the marine green alga Ostreococcus. Proc. Natl. Acad. Sci. U. S. A. 105:7881-7886 (2008)(Pubmed)
P. F. Devaux, A. Herrmann, N. Ohlwein and M. M. Kozlov. How lipid flippases can modulate membrane structure. Biochim. Biophys. Acta 1778:1591-1600 (2008)(Pubmed)
Y. Gohon, T. Dahmane, R. W. H. Ruigrok, P. Schuck, D. Charvolin, F. Rappaport, P. Timmins, D. M. Engelman, C. Tribet, J.-L. Popot and C. Ebel. Bacteriorhodopsin/amphipol complexes: structural and functional properties. Biophys. J. 94:3523-3537 (2008)(Pubmed)

2007

A. Papadopulos, S. Vehring, I. Lopez-Montero, L. Kutschenko, M. Stockl, P. F. Devaux, M. Kozlov, T. Pomorski and A. Herrmann. Flippase activity detected with unlabeled lipids by shape changes of giant unilamellar vesicles. J. Biol. Chem. 282:15559-15568 (2007)(Pubmed)
C. Diab, C. Tribet, Y. Gohon, J-L. Popot and F. M. Winnik. Complexation of integral membrane proteins by phosphorylcholine-based amphipols. Biochim. Biophys. Acta 1768:2737-2747 (2007)(Pubmed)
D. P. Yakubu, A. Mostyn, V. Wilson, S. Pearce, M. C. Alves-Guerra, C. Pecqueur, B. Miroux, H. Budge, T. Stephenson and M. E. Symonds. Different effects of maternal parity, cold exposure and nutrient restriction in late pregnancy on the abundance of mitochondrial proteins in the kidney, liver and lung of postnatal sheep. Reproduction 133:1241-1252 (2007)(Pubmed)
E. Chevillotte, M. Giralt, B. Miroux, D. Ricquier and F. Villarroya. Uncoupling protein-2 controls adiponectin gene expression in adipose tissue through the modulation of reactive oxygen species production. Diabetes 56:1042-1050 (2007)(Pubmed)
F. Baymann, F. Giusti, D. Picot and W. Nitschke. The ci/bH moiety in the b6f complex studied by EPR: a pair of strongly interacting hemes. Proc. Natl. Acad. Sci. U. S. A. 104:519-524 (2007)(Pubmed)
I. Lopez-Montero, M. Velez and P. F. Devaux. Surface tension induced by sphingomyelin to ceramide conversion in lipid membranes. Biochim. Biophys. Acta 1768:553-561 (2007)(Pubmed)
K. Moncoq, C. A. Trieber and H. S. Young. The molecular basis for cyclopiazonic acid inhibition of the sarcoplasmic reticulum calcium pump. J. Biol. Chem. 282:9748-9757 (2007)(Pubmed)
K.-H. Park, C. Berrier, F. Lebaupain, B. Pucci, J.-L. Popot, A. Ghazi and F. Zito. Fluorinated and hemifluorinated surfactants as alternatives to detergents for membrane protein cell-free synthesis. Biochem. J. 403:183-187 (2007)(Pubmed)
M. Flotenmeyer, H. Weiss, C. Tribet, J.-L. Popot and K. Leonard. The use of amphipathic polymers for cryo electron microscopy of NADH:ubiquinone oxidoreductase (complex I). J. Microsc. 227:229-235 (2007)(Pubmed)
M. Zoonens, F. Giusti, F. Zito and J.-L. Popot. Dynamics of membrane protein/amphipol association studied by Forster resonance energy transfer: implications for in vitro studies of amphipol-stabilized membrane proteins. Biochemistry 46:10392-10404 (2007)(Pubmed)
N. Bensalem, S. Masscheleyn, J. Mozo, B. Vallee, F. Brouillard, S. Trudel, D. Ricquier, A. Edelman, I. Chiara Guerrera and B. Miroux. High sensitivity identification of membrane proteins by MALDI TOF-MASS spectrometry using polystyrene beads. J. Proteome Res. 6:1595-1602 (2007)(Pubmed)
V. Petkova, J.-J. Benattar, M. Zoonens, F. Zito, J.-L. Popot, A. Polidori, S. Jasseron and B. Pucci. Free-standing films of fluorinated surfactants as 2D matrices for organizing detergent-solubilized membrane proteins. Langmuir 23:4303-4309 (2007)(Pubmed)
V. S. Tran, S. Huet, I. Fanget, S. Cribier, J.-P. Henry and E. Karatekin. Characterization of sequential exocytosis in a human neuroendocrine cell line using evanescent wave microscopy and "virtual trajectory" analysis. Eur. Biophys. J. 37:55-69 (2007)(Pubmed)