Welcome to the Laboratory of Physical and Chemical Biology of Membrane Proteins

CNRS UMR 7099 - University Paris Diderot - Paris 7

Our laboratory gathers biologists, physicists and chemists who are interested in the structure, structural dynamics and physical chemistry of membrane proteins, either in vivo within a lipid bilayer, or in vitro after solubilization and stabilization by classical or alternative surfactants such as amphipols.

Our proteins of interest cover various domains of fundamental and medical biology:

  • Energy coupling & supramolecular organization of respiratory chain complexes with the structure-function study of the cytochrome b6f complex and the mitochondrial uncoupling proteins.
  • Molecular Signalization Pathway of GPGRs with the study of HEDGEHOG Pathway, the study of Signal Transduction by GPCRs using liquid state NMR and GPCR Cristallization.
  • Transport and Membrane Dynamics in Bacteria including the biochemistry of Iron Transport in Haemophilus, the membrane biogenesis in E. coli, solid state NMR of membranes and membrane proteins and the study of efflux pumps in bacteria.

In addition, all members of the unit contribute to the development of our tool box for membrane proteins studies, which includes: NMR, mass spectrometry, crystallography, physical-chemistry of surfactants, membrane protein production and synthetic biology.


Bruno Miroux

Head of unit

Recently published

A complete and up to date coverage on Membrane Proteins in Aqueous Solutions, Springer International Publisher, 2018, written by Jean-Luc Popot is now available: Web link

C. Le Bon, A. Marconnet, S. Masscheleyn, J.-L. Popot & M. Zoonens (2018) Folding and stabilizing membrane proteins in amphipol A8-35. Methods doi: 10.1016/j.ymeth.2018.04.012. [Epub ahead of print](Pubmed)

M.V. Dilworth, M.S. Piel, K.E. Bettaney, P. Ma, J. Luo, D. Sharples, D.R. Poyner, S.R. Gross, K. Moncoq, P.J.F. Henderson, B. Miroux & R.M. Bill (2018) Microbial expression systems for membrane proteins. Methods doi: 10.1016/j.ymeth.2018.04.009. [Epub ahead of print](Pubmed)

M. Casiraghi, M. Damian,E. Lescop, J.-L. Baneres & L.J. Catoire (2018) Illuminating the energy landscape of GPCRs: the key contribution of solution-state NMR associated with Escherichia coli as an expression host. Biochemistry. doi: 10.1021/acs.biochem.8b00035. [Epub ahead of print](Pubmed)

Membranes & Molecules Seminars

Thibaud Renault
(Wednesday, April 11th 2018, 14h00)
Max Planck Institute for Infection Biology, Berlin, Germany
Humboldt Universität zu Berlin, Berlin, Germany

Self-Assembly of a Bacterial Nanomachine: the Flagellum (abstract)

Workshop

The traditionnal meeting 'ABC of membrane transport' will take place on Friday October 20th 2017 in the Bibliothèque of IBPC, Paris. Speakers for keynote lectures: Muriel Masi/Ben Luisi/Nicolas Reyes /Marc Boutry. (Program).

PhD defense

Federica Angius, October 13th 2017
"Molecular basis of membrane protein production and intracellular membranes proliferation in E. coli"(abstract)

Education

Structural Biochemistry Course IBPC:
From Protein Identification by Mass Spectrometry to Crystal Structure Determination and Molecular Dynamic Analysis.

---From 8th to 16th March 2018---
More details and Program.

Course Integrative Structural Biology​
--Mechanisms of Living Organisms--

---From 17th to 22th July 2017---
More details and Program.

Job position

No position available for the moment.

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Last update: 18/04/04

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