Welcome to the Laboratory of Physical and Chemical Biology of Membrane Proteins

CNRS UMR 7099 - University Paris Diderot - Paris 7

Our laboratory gathers biologists, physicists and chemists who are interested in the structure, structural dynamics and physical chemistry of membrane proteins, either in vivo within a lipid bilayer, or in vitro after solubilization and stabilization by classical or alternative surfactants such as amphipols.

Our proteins of interest cover various domains of fundamental and medical biology:

  • Energy coupling & supramolecular organization of respiratory chain complexes with the structure-function study of the cytochrome b6f complex and the mitochondrial uncoupling proteins.
  • Molecular Signalization Pathway of GPGRs with the study of HEDGEHOG Pathway, the study of Signal Transduction by GPCRs using liquid state NMR and GPCR Cristallization.
  • Transport and Membrane Dynamics in Bacteria including the biochemistry of Iron Transport in Haemophilus, the membrane biogenesis in E. coli, solid state NMR of membranes and membrane proteins and the study of efflux pumps in bacteria.

In addition, all members of the unit contribute to the development of our tool box for membrane proteins studies, which includes: NMR, mass spectrometry, crystallography, physical-chemistry of surfactants, membrane protein production and synthetic biology.


Bruno Miroux

Head of unit

Recently published

A complete and up to date coverage on Membrane Proteins in Aqueous Solutions, Springer International Publisher, 2018, written by Jean-Luc Popot is now available: Web link

F. Angius, O. Ilioaia, A. Amrani, A. Suisse, L. Rosset, A. Legrand, A. Abou-Hamdan, M. Uzan, F. Zito & B. Miroux (2018) A novel regulation mechanism of the T7 RNA polymerase based expression system improves overproduction and folding of membrane proteins. Sci Rep. 8(1):8572. Pubmed)

M. Serra-Batiste, J. Tolchard, F. Giusti, M. Zoonens & N. Carulla (2018) Stabilization of a Membrane-Associated Amyloid-β Oligomer for Its Validation in Alzheimer's Disease. Front Mol Biosci. 5:38. doi: 10.3389/fmolb.2018.00038. eCollection 2018 (Pubmed)

L. Dumas, F. Zito, P. Auroy, X. Johnson, G. Peltier & J. Alric (2018) Structure-function analysis of chloroplast proteins via random mutagenesis using error-prone PCR. Plant Physiol. doi: 10.1104/pp.17.01618. [Epub ahead of print](Pubmed)

Membranes & Molecules Seminars

Mickaël Blaise
(Thursday, June 28th 2018, 11h30)
Institut de Recherche en Infectiologie de Montpellier (IRIM),
UMR9004-CNRS, université de Montpellier

Molecular insights into the versatile roles of Mycobacterial Membrane Protein Large: from lipid transport to drug resistance and pathogenicity (abstract)

Workshop

The traditionnal meeting 'ABC of membrane transport' will take place on Friday October 20th 2017 in the Bibliothèque of IBPC, Paris. Speakers for keynote lectures: Muriel Masi/Ben Luisi/Nicolas Reyes /Marc Boutry. (Program).

PhD defense

Federica Angius, October 13th 2017
"Molecular basis of membrane protein production and intracellular membranes proliferation in E. coli"(abstract)

Education

Structural Biochemistry Course IBPC:
From Protein Identification by Mass Spectrometry to Crystal Structure Determination and Molecular Dynamic Analysis.

---From 8th to 16th March 2018---
More details and Program.

Course Integrative Structural Biology​
--Mechanisms of Living Organisms--

---From 16th to 21th July 2018---
More details and Program.

Job position

No position available for the moment.

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Last update: 18/06/13

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